结核分枝杆菌19 kDa脂蛋白诱导THP-1细胞凋亡的研究  

Study on apoptosis of THP-1 cells induced by Mycobacterium tuberculosis 19 kDa lipoprotein

作　　者：韦莉[1] 金齐力[1] 刘勇[1] 夏佩莹[1] 

机构地区：[1]蚌埠医学院病原生物学教研室安徽省感染与免疫重点实验室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2008年第3期263-265,共3页Journal of Bengbu Medical College

基　　金：安徽省高校省级自然科学研究计划项目(KJ2008B320);安徽省省级重点实验室重点科研项目(2004sys008)

摘　　要：目的:观察结核分枝杆菌19 kDa脂蛋白(Mtb P19)对THP-1细胞凋亡的影响。方法:从培养的结核杆菌H37Ra制备Mtb P19,以5、10、20μg/ml Mtb P19作用于佛波醇酯(PMA)分化的THP-1细胞,37℃、5%CO2孵箱孵育4 h。Wright-Giemsa染色观察细胞形态变化;AnnexinV-FITC染色,用流式细胞仪检测细胞凋亡。结果:终浓度为5μg/ml的Mtb P19即可诱导THP-1细胞凋亡,且凋亡率随Mtb P19作用浓度的升高而增加(P<0.01);Wright-Giemsa染色镜下可见部分细胞体积变小、圆缩,核固缩,核断裂。结论:结核分枝杆菌P19以剂量依赖方式诱导THP-1细胞凋亡。Objective：To abserve the influence of Mycobacterium tuberculosis 19 kDa（ Mtb P19 ） on the apoptosis of THP-1 cells. Methods：Mtb P19 was prepared from cultured Mtb H37Ra. Phorbol myristate acetate-differentiated THP-1 cells were incubated with P19 at the concentrations from 5 μg/ml to 20 μg/ml at 37 ℃ ,5% CO2 for up to 4 hours. The change of cells was observed by WrightGiemsa stain,and the apoptosis rate was detected by flowcytometry. Results： Mtb P19 could induce THP-1 cells apoptosis at final concentration of 5 μg/ml,and the apopotosis rate increased with the concentration of P19 （P 〈 0.01 ）. Round shrinkage,pyknosis or nuclear fragmentation were observed in some cells by Wright-Giemsa stain. Conclusions：Mtb P19 induces apoptosis in THP-1 cells in a dose-dependent manner.

关 键 词：细胞凋亡 结核分枝杆菌19kDa脂蛋白 THP-1细胞 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学]