难免流产蜕膜组织遗传印记基因PEG10的表达  

Genetic imprinted gene PEG10 expression in deciduas from inevitable abortion

作　　者：梁小妍[1] 刘学庆[1] 丁裕斌[1] 陈雪梅[1] 王应雄[1] 何俊琳[1] 

机构地区：[1]重庆医科大学公共卫生学院生殖生物学研究室,遗传优生教研室,重庆400016

出　　处：《遗传》2008年第6期735-740,共6页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30500054);重庆市科委重点项目(渝科发计字[2004]47号)资助~~

摘　　要：采用半定量逆转录聚合酶反应(RT-PCR)、原位杂交、免疫印记(Western blot)及免疫组织化学技术检测了36例难免流产患者蜕膜组织PEG10(Paternally expressed gene10)mRNA及蛋白的表达与分布,并以36例同期正常早孕妇女为对照,研究遗传印记基因在难免流产蜕膜组织中的表达,探讨其在自然流产中的作用。RT-PCR结果显示,PEG10在两组蜕膜组织中均有表达,正常妊娠组平均表达水平为0.5994±0.049,难免流产组为0.1783±0.037,两组比较具有显著性差异(P<0.05)。原位杂交、免疫组化及Western blot分析也显示PEG10的表达规律与RT-PCR结果相吻合。研究结果表明,遗传印记基因PEG10维持一定水平的表达对早期胚胎发育和正常妊娠的维持有重要意义,而其表达下调可能是导致难免流产的原因之一。The expression and distribution of the imprinted paternally expressed gene 10 （PEG10） in decidual tissues of 36 patients with inevitable abortion and 36 normal pregnant （NP） women were examined by RT-PCR, immunohistochemistry, in situ hybridization and Western blot. Decidual PEGIO mRNA expression was significantly lower in the patients than that in the NP group by RT-PCR （P〈0.05）. The characteristic PEG10 expression was confirmed by in situ hybridization. As shown by Western blot and immunohistochemistry, PEG10 protein expression was consistent with the mRNA expression pattern. The results showed that maintaining sufficient levels of PEGIO may be important during early pregnancy. Lower levels of PEG10 might be one of reasons that underlie inevitable abortion.

关 键 词：遗传印记 PEG10 蜕膜 难免流产 

分 类 号：R714.21[医药卫生—妇产科学]