ES小鼠和ES细胞中H19基因甲基化状态  

Methylation of H19 gene in ES mice and embryonic stem cells

作　　者：杨荣荣[1] 周荣艳[1] 高淑敏[1] 温晓辉[1] 李相运[1] 

机构地区：[1]河北农业大学动物科技学院,河北省牛羊胚胎工程技术研究中心,保定071001

出　　处：《遗传》2008年第6期765-770,共6页Hereditas（Beijing)

基　　金：国家自然科学基金项目(No.30571336);河北农业大学校长基金项目资助~~

摘　　要：为探讨印迹基因H19的甲基化状态与ES小鼠胚胎发育之间的关系,以遗传背景相同的正常成年对照小鼠、22只成年ES小鼠和8只新生死亡的ES小鼠以及不同传代次数的ES细胞为实验材料,利用甲基化敏感性限制性内切酶-PCR技术分别检测了其印迹基因H19的5′非翻译区两个位点的甲基化状态。结果表明,发育至成年的ES小鼠印迹基因H19所检测位点的甲基化状态与正常成年对照小鼠之间没有差异,而新生死亡的ES小鼠印迹基因H19所检测位点的甲基化状态与成年ES小鼠以及正常成年对照小鼠相比则存在明显差异。推测ES细胞中印迹基因H19所检测位点的甲基化状态与成年ES小鼠以及正常成年对照小鼠之间可能存在差异。To investigate the relationship between the methylation status of imprinted gene H19 and development of mice derived completely from Embryonic stem cells （ES） by tetraploid embryo complementation. The methylation status of two loci at 5＇UTR region in imprinted gene H19 in normal adult control mice, 22 adult ES mice, 8 newborn dead ES mice and embryonic stem （ES） cells with different passage number were detected by the using of methylation-sensitive restriction endonuclease-PCR technique. The results indicated that the methylation status of imprinted gene H19 in adult ES mice were identical to that of normal adult control mice. However, some significant differences in the methylation status of imprinted gene H19 were found among newborn dead ES mice, adult ES mice and normal adult control mice. Furthermore, the methylation status of imprinted gene H19 in ES cells were probably different from that of adult ES mice and normal adult control mice.

关 键 词：ES细胞 ES小鼠 DNA甲基化 印迹基因H19 

分 类 号：Q343[生物学—遗传学]