元江普通野生稻叶片cDNA文库的构建及部分基因片段分析  

Construction and analysis of cDNA library of Yunnan Yuanjiang O.rifupogon leaf

作　　者：史冬燕[1] 杨明挚[1] 黄兴奇[2] 陈善娜[1] 程在全[2] 

机构地区：[1]云南大学生命科学学院 [2]云南省农科院生物技术与种质资源研究所,昆明650203

出　　处：《遗传》2008年第6期776-780,共5页Hereditas（Beijing)

基　　金：国家自然科学基金项目(编号:30460019);云南省重点基金项目(编号:2004C0010Z);云南省科技攻关项目(编号:2006NG34)资助~~

摘　　要：首次利用SMARTTM技术构建了中国普通野生稻中最原始类型——元江普通野生稻生长旺盛时期叶片的cDNA文库。该cDNA文库未扩增和扩增后的滴度分别为1.1×106pfu/mL和3.98×107pfu/mL,重组率为91%,插入片段大小为500～2000bp。测定的部分cDNA序列进行BLAST比较,发现这些cDNA片段与日本晴栽培稻同源性很高,达到98%以上。本研究为进一步分析这些cDNA片段的结构、功能和探讨元江普通野生稻在栽培稻演化中的地位奠定了基础。The cDNA library of Yuanjiang Oryza rifupongon leaf was constructed by using SMARTTM technology. The titers of the non-amplified library and the amplified library were 1.1 × 10^6 pfu/mL and 3.98 × 10^7 pfu/mL, respectively. The recombination rate was more than 91%. The DNA sequence length of the most cDNAs in the library was between 500-2 000 bp. Some cDNAs chosen by random were sequenced. After BLAST analysis of some cDNAs, their possible function were predicted. It is found that these cDNAs show 98% similarity to Oryza sativa japonica in the NCBI database. These provided a base for further study on the structure and function of these cDNAs and evolutionary process of Yuanjiang Oryza rifupongon.

关 键 词：CDNA文库 元江普通野生稻 滴度 

分 类 号：S511.9[农业科学—作物学]