利用两种方法构建的近等基因系对水稻两个多效区段遗传效应进行评价  

Two pleiotropic intervals of rice were assessed using NILs constructed by two methods

作　　者：张玉山[1] 吴薇[1] 徐才国[2] 

机构地区：[1]电子科技大学中山学院,中山528402 [2]华中农业大学作物遗传改良国家重点实验室,武汉430070

出　　处：《遗传》2008年第6期781-787,共7页Hereditas（Beijing)

基　　金：国家重点基础研究发展规划项目(编号:G1998010200);电子科技大学中山学院博士启动基金资助项目资助~~

摘　　要：水稻每穗颖花数是水稻产量的重要构成因子之一。适当的抽穗期和株高对水稻高产是非常必要的。依据珍汕97和HR5衍生的重组自交系初步定位的结果,利用高世代回交的方法构建了第7染色体同时控制抽穗期、株高和每穗颖花数的靶区段近等基因系(BC4F2);利用基于重组自交系群体的杂合区段自交的方法构建了第8染色体同时控制抽穗期、株高和每穗颖花数的靶区段近等基因系,并利用两个近等基因系对这两个多效区段的遗传效应进行了准确的评价。两个近等基因系的QTL分析结果表明,3个性状都是由一个QTL或紧密连锁的QTL控制,而且加性效应和显性效应的方向均相同;同时3个性状在各自的近等基因系中呈现典型的双峰分布或不连续分布,这些结果暗示3个性状可能是一因多效的结果。文章还对抽穗期和株高与水稻产量的关系、3个性状显著正相关在育种中的应用及两种构建近等基因系方法的优缺点也进行了讨论。Spikeletes per panicle （SPP） is one of the important yield components in rice （Oryza sativa L.）. Appropriate heading date （HD） and plant height （PH） are prerequisites for desired yield. In this study, the near isogenic lines （NILs） of chromosome 7, which harbored QTLs controlling HD, PH and SPP were constructed based on the primary QTLs results of the recombinant inbred lines （RILs） derived from Zhenshan 97 and HR5. The near isogenic lines （RILs） of chromosome 8, which harbored QTLs of HD, PH, and SPP, were constructed using heterogeneous inbred family based on RILs. The genetic effects of two pleiotropic intervals were assessed accurately in these two NILs. The QTLs identified by the two near isogenic lines indicated that the three traits were controlled by the same QTL or tightly linked QTL. The QTLs controlling the three traits had the same direction on additive effects and dominant effects. And these traits showed bimodal or discontinuous distributions in respective N1Ls. These results imply that the same gene might control the three traits simultaneously （i.e., the gene pleiotropism）. Along with this, the relationships between HD and PH and yield, the application of significantly positive correlation for the three traits in breeding program, and the advantage and disadvantage of the two constructing NILs methods were also discussed in this study.

关 键 词：每穗颖花数 株高 抽穗期 重组自交系 近等基因系 水稻 珍汕97 HR5 

分 类 号：S511[农业科学—作物学]