重症急性胰腺炎大鼠模型制作方法的改进  

Technique improvement on traditional animal model with severe acute pancreatitis in rats

作　　者：陈占峰[1] 陈启龙[1] 

机构地区：[1]新疆医科大学第一附属医院普外科,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2008年第4期461-462,465,共3页Journal of Xinjiang Medical University

摘　　要：目的:探讨4%牛磺胆酸钠逆行胆胰管注射诱导重症急性胰腺炎(SAP)大鼠模型的相关技术的改良。方法:将36只健康SD大鼠随机分为模型组(n=18)和假手术组(n=18),应用细针经胆胰管近十二指肠开口端逆行刺入注射4%牛磺胆酸钠,诱导重症急性胰腺炎动物模型;2组均在模型诱导后3、6、12h后检测腹水量及血淀粉酶,观察胰腺组织形态变化,光镜下进行胰腺病变程度评分。结果:模型组在模型诱导后3、6、12h腹水量、血淀粉酶、胰腺组织学评分均较假手术组明显升高(P<0.05)。结论:该方法可成功诱导重症急性胰腺炎,具有创伤小、操作简便及模型诱导成功率高的特点,是较为理想的SAP动物模型。Objective： To study related technique improvement on traditional animal model with severe acute pancreatitis in rats. Methods： The animals were randomly divided into experimental or shame operation group. Severe acute pancreatitis animal model was induced retrogradely by injected of 4% sodium taurocholate into biliopancreatic ducts with fine needle. Blood serum amylase activity and the amount of ascites were obtained at 3 hours, 6 hours, 12 hours respectively. Tissue samples of pancreas were prepared for morphological observation under conventional light microscopy. Pathological change of pancreas was evaluated by Kusske score system. Results： The concentration of serum amylase, the amount of ascites and pathological score in model group were significantly higher than that in shame operation group （P 〈0.05）. Conclusion： Severe acute pancreatitis model is successfully induced by this method. It minimizes the trauma and simplized the procedures. It might be a reliable model with high success rate and an ideal severe acute pancreatitis animal model for recommendation.

关 键 词：重症急性胰腺炎 动物模型 牛磺胆酸钠 技术改进 

分 类 号：R657.5[医药卫生—外科学] R-332[医药卫生—临床医学]