HIV-1B′特异性细胞毒性T细胞与疾病进展关系  

Relationship between HIV-1 B′specific cytotoxic T lymphocytes and disease progression

作　　者：崔华露[1] 耿文清[1] 姜拥军[1] 韩晓旭[1] 潘莹[1] 年华[1] 包名家[1] 王亚婷[1] 尚红[1] 

机构地区：[1]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中国公共卫生》2008年第6期691-693,共3页Chinese Journal of Public Health

基　　金：卫生部艾滋病防治应用性研究项目(WA2006-02);国家重点基础研究发展计划(973计划)项目(2006CB504206);辽宁省科技攻关项目(2005225001)

摘　　要：目的了解人类免疫缺陷病毒I型(HIV-1)B′亚型特异性细胞毒性T细胞(CTL)功能与中国HIV-1感染者疾病进展关系。方法将覆盖HIV-1B′亚型Gag p17、p24和p2p7plp6全长的54个重叠多肽作为抗原,用酶联免疫斑点实验(ELISPOT)检测58例HIV-1感染者特异性CTL对上述多肽的应答情况。结果中国HIV-1感染者特异性CTL可识别多个HIV-1B′Gag表位,反应宽度与病毒载量显著负相关(r=-0.374,P=0.004),与CD4+T细胞绝对计数显著正相关(r=0.425,P=0.001),反应强度与病毒载量显著负相关(r=-0.285,P=0.030)。长期不进展者识别HIV-1B′Gag多肽的反应宽度显著高于无症状感染者和艾滋病患者(P=0.001,P=0.005)。结论我国HIV-1感染者体内存在识别不同HIV-1B′Gag多肽表位的特异性CTL应答,并且与疾病进展相关。Objective To investigate relationship between human immunodeficiency virus type I （HIV- 1 ）B＇ specific cytotoxic T lymphocytes （CTL） and disease progressior/in Chinese HIV - 1 infected individuals. Methods We stimulated HIV- 1B＇ Gag specific CTL isolated from 58 HIV- 1 infected individuals, with 54 overlapping peptides which were span- ning the entire Gag protein of HIV- 1 clade B＇, then determined function of CTL using an Enzyme - Linked Immunospot （ELISPOT） assay. Results There were varieties of epitopes that were recognized by specific CTL in Chinese HIV- 1 infected individuals. The breadth and magnitude of CTL response were reversly correlated with the HIV - 1 viral load significantly（ r = - 0. 374, P = 0. 004 ; r = - 0. 285, P = 0. 030）, the breadth of CTL response was correlated with the true counts of CD4 ＋ T lymphocytes significantly（ r = 0. 425, P = 0.001 ）. The breadth of CTL response of long term non progressors was significantly superior to that of asymptomatic HIV infected and acquired immunodeficiency syndrome（AIDS） patients（ P = 0.001;P = 0.005）. Conclusion There are specific CTL that response against different HIV- 1B＇ Gag peptides in Chinese HIV- 1 infected individuals and the function of CTL are correlated with disease progression.

关 键 词：细胞毒性T细胞(CTL) 人类免疫缺陷病毒I型(HIV-1)B′亚型 酶联免疫斑点实验(ELISPOT) 疾病进展 

分 类 号：R512.91[医药卫生—内科学] Q256[医药卫生—临床医学]