新疆地区汉族和维吾尔族人群感染幽门螺杆菌cagA分布特征及与胃相关性疾病的关系  

The characters and relationship between the cagA distribution of helicobacter pylori to gastric diseases among the Uygur and Han populations in Xinjiang

作　　者：许海峰[1] 王亚男[2] 布海里切木.吾甫尔 刘素辉[1] 德里夏提.依米提 

机构地区：[1]新疆医科大学基础医学院微生物学教研室 [2]新疆医科大学基础医学院形态中心 [3]新疆维吾尔自治区人民医院消化科,新疆乌鲁木齐830000

出　　处：《新疆医科大学学报》2008年第5期521-523,共3页Journal of Xinjiang Medical University

基　　金：新疆维吾尔自治区科技厅自然科学基金资助项目(200521109)

摘　　要：目的:探讨新疆地区汉族和维吾尔族慢性浅表性胃炎、慢性萎缩性胃炎、胃溃疡、胃癌患者感染幽门螺杆菌(H.pylori)细胞毒素相关基因(cagA)的分布特征及与胃相关性疾病的关系。方法:从92例胃相关性疾病的患者(汉族46例、维吾尔族46例)胃镜活检标本中分离培养H.pylori,抽提各菌株的总DNA,采用特异性引物进行PCR扩增检测H.pylori的cagA基因,并分析其分布特征及与胃相关性疾病的关系。结果:92株H.pylori菌株中cagA基因的检出率为84.8%,在所研究的4种疾病中汉族和维吾尔族患者感染的H.pylori cagA基因检出率差异无统计学意义(P>0.05);汉族和维吾尔族患者4种特定疾病的H.pylori cagA基因检出率差异无统计学意义(P>0.05)。结论:新疆地区汉族和维吾尔族间H.pylori cagA基因阳性率未见明显差异,特定胃相关性疾病间H.pylori cagA基因阳性率也未见明显差异。Objective： To investigate the prevalence of cytotoxin associated gene （cagA） of Helicobacter pylori （H. pylori） in patients with upper gastroic disease among the Uygur and Han populations in Xinjiang area and to assess the relationship between cagA and H. pylori related gastrointestinal disease. Methods： Totally 92 H. pylori strains were isolated from patients with chronic gastroic disease in Xinjiang. Bacterial DNA from all these strains was extracted. The prevalence and genetic diversity in cagA sequence were studied by the technique of polymerase chain reaction （PCR）. Results： In Xinjiang area, H. pylori cagA positive rate was 84.8% （78/92）, There was no significantly difference in cagA gene between Han population and Uygur population （P 〉0.05） and there was no significantly difference in cagA gene between different gastric diseases （P〈0.05）. Conclusion： Positive rate of cagA was not significantly different between special Han population and Uygur population and between special chronic gastric diseases in Xinjiang area.

关 键 词：幽门螺杆菌 CAGA基因 聚合酶链反应 

分 类 号：R378.2[医药卫生—病原生物学]