基于生物质谱的蛋白质组学绝对定量方法研究进展  

Development of Absolute Quantification of Proteomics Based on Bio-Mass Spectrum

作　　者：曹冬[1] 张养军[1] 钱小红[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京蛋白质组研究中心,北京102206

出　　处：《质谱学报》2008年第3期185-191,共7页Journal of Chinese Mass Spectrometry Society

基　　金：国家重点基础研究规划项目(2001CB510201,2004CB520802);国家科技计划课题“蛋白质科学计划973基地项目”(2006CB910803);国家自然科学基金(30321003、20405017、20505018、20505019);北京市科委重大项目(H030230280190)资助

摘　　要：蛋白质组学定量方法包括相对定量和绝对定量两部分。相对定量蛋白质组学(也称比较蛋白质组学)是指对不同生理病理状态下细胞、组织或体液蛋白质表达量的相对变化进行比较分析;绝对定量蛋白质组学是测定细胞、组织或体液蛋白质组中每种蛋白质的绝对量或浓度。目前蛋白质组学的绝对定量方法主要有基于内标法的蛋白质组学绝对定量方法和基于质谱数据统计分析的非标记方法。蛋白质组学定量的信息可以帮助了解蛋白质在相互作用网络中所起的作用;通过对临床生物标记物绝对量的分析,可以帮助直观地判断疾病的发生和发展过程,这对于临床诊断和疾病治疗都具有现实的指导意义。The quantitative methods of proteomics include the relative quantitation and absolute quantitation of proteins. Relative quantitation of proteomics is the analysis of the relative changes of the expressed proteins in the samples originated from different physiological and pathological situations. The absolute quantitation of proteomics is to determine the absolute amount or concentration of each protein in a biological sample, such as cells,tissue or body fluid. Currently, the main strategies of absolute quantitation of proteomics are composed of the quantitative methods based on internal standard strategy and the label-free technique with the statistic analysis of MS data. The quantitative information of the proteome can help us understand the biological function of proteins in the interaction network. With the determination of the absolute quantitation of the biomarker, we can directly judge the status of a disease, which will benefit the clinical diagnosis and the treatment of the disease.

关 键 词：定量蛋白质组 绝对定量 同位素标记法 非标记定量法 生物质谱 

分 类 号：O657.63[理学—分析化学] Q1[理学—化学]