次氮三乙酸铁对大鼠糖代谢影响的研究  

A Study of the Effect of Ferric Nitrilotriacetate on Glycometabolism in the Wistar Rats

作　　者：陈敏[1] 田浩明[1] 杨波[1] 赵桂芝[1] 张翔迅[1] 王嘉南[1] 

机构地区：[1]四川大学华西医院内分泌科

出　　处：《川北医学院学报》2008年第3期222-225,共4页Journal of North Sichuan Medical College

摘　　要：目的了解体内铁超负荷对大鼠糖代谢的影响。方法雄性Wistar大鼠随机分四组:铁干预组(A组)、次氮三乙酸二钠对照组(B组)、铁加去铁干预组(C组)、空白对照组(D组)。观察腹腔葡萄糖耐量试验(IPGTT),测定血清铁(SI)、血清铁蛋白(SF)、血清丙二醛(MDA)变化。取胰腺作铁染色行积分光密度(IOD)值分析。结果(1)A、C组大鼠体重增长明显低于B、D组(p值<0.05)。(2)A、C组SI、SF、MDA、胰腺铁染色IOD值显著高于B、D组(p值<0.05)。(3)A、C组胰腺组织可见普鲁士蓝颗粒沉积。(4)第10周时A、C组基础血糖及2小时血糖较B、D组高(p=0.059,p=0.043),A组2小时胰岛素水平有降低趋势;干预后A、C组基础血糖及2小时血糖较干预前高(p<0.05)。结论(1)给Wistar大鼠反复腹腔注射FeNTA八周后建立铁超负荷大鼠模型。(2)铁超负荷大鼠胰腺组织可见铁沉积。(3)铁超负荷大鼠脂质过氧化产物明显增高。(4)铁超负荷大鼠糖耐量受损,β细胞分泌胰岛素能力有降低趋势。Objective To explore the effect of iron overload on glycometabolism of the Wistar rats. Methods Wistar male rats were divided randomly into four groups. Group A received FeNTA. Group B received Na2NTA, Group C received DETAPAC and FeNTA . Group D was untreated control. Intraperitoneal glucose tolerance tests （IPGTT） were performed. Serum iron （SI） , serum ferritin （SF） , and serum malondialdehyde （MDA） were measured. Portions of pancreas of each rat were collected for Prussian blue stain. The values of IOD of iron granules of pancreas were analyzed . Results （ 1 ） Group A and C showed a lower growth rate than group B and D （p 〈0. 05）. （2） The levels of SI,SF,MDA,IOD of iron granules in group A and C were higher than group B and D （p 〈0. 05）. （3） Prussian blue positive granules were observed in parenchymal cells of pancreas in group A and C. （4） At lOth week, the basal plasma glucose （BPG） and plasma glucose 2 hours after the glucose load （2hPG） of group A and C were higher than group B and D（p = 0. 059, p = 0. 043 ） , and the plasma insulin of group A had a tendency to decrease ; Group A and C showed elevated levels of BPG and 2hPG after intervention （p 〈 0. 05 ）. Conclusions （ 1 ） An iron overload rat model was developed by repeated injections（ i. p. ） of FeNTA for 8 weeks. （2） Iron granules were found to deposit in pancreas of the model. （ 3 ） Iron overload rats had higher level of lipid peroxidation in vivo. （4） Iron overload in vivo impaired glucose tolerlance and capacity of β cells secrete insulin.

关 键 词：大鼠 铁超负荷 糖代谢 氧化应激 

分 类 号：R333.6[医药卫生—人体生理学]