细胞感染法与聚合酶链式反应法对热力灭活病毒效果评价的研究  

STUDY ON EVALUATION OF EFFICACY OF HEAT IN INACTIVATING VIRUSES BY CELL INFECTION METHOD AND PCR METHOD

作　　者：许金波[1] 刘育京[1] 李成文[1] 张文福[1] 杨瑞馥[1] 

机构地区：[1]军事医学科学院微生物流行病研究所

出　　处：《中国消毒学杂志》1997年第4期198-203,共6页Chinese Journal of Disinfection

摘　　要：水泡性口炎病毒（ＶＳＶ）经８０℃２小时和１００℃３０分钟干热灭活后，其细胞感染试验与ＰＣＲ分析结果并不一致。细胞感染试验为阴性，但用ＰＣＲ方法却仍能检出ＶＳＶ的核衣壳基因片段。这种结果提示，在一定条件下，病毒核酸的热变性可能不是断裂，或断点不在扩增片段范围之内。故用ＰＣＲ方法评价热力灭活病毒效果时应慎重。n order to know the difference of evaluation of inactivation efficacy by cell infection method and PCR method in virus inactivation test, test for inactivation of vesicular stomatitis virus (VSV) by heat was used for observation and comparison of these 2 methods.The results indicated that after dry heat inactivation of VSV at 80℃ for 2 hr and 100℃ for 30 min, the cell infection test was negative, while PCR could still detect fragments of nuclear capsid gene. When dosage of heat was increased to 80℃ for 72 hr or 100℃ for 2 hr, PCR test was still positive. This indicates that under certain conditions, heat denaturalization of viral RNA may be not fragmentation or the break site not located in amplification fragment, therefore it should be careful to use PCR method for evaluating the efficacy of heat in inactivating viruses.

关 键 词：细胞感染法 病毒 VSV 灭活效果 PCR 

分 类 号：R187.5[医药卫生—流行病学] R373[医药卫生—公共卫生与预防医学]