龟板抗Parkinson病大鼠多巴胺能神经元凋亡的作用  

Protective effect of Plastrum Testudinis on the apoptosis of dopamine neurons of rats with Parkinson's disease

作　　者：邓汝东[1] 李伊为[1] 陈东风[1] 黎晖[1] 张赛霞[1] 赵丹[1] 孟令杰[1] 周健洪[1] 盂冬雪 张瑞[1] 张海玲[1] 

机构地区：[1]广州中医药大学解剖学教研室,广州510006

出　　处：《神经解剖学杂志》2008年第3期301-306,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(Nos30472272;30772861)资助项目

摘　　要：为探讨补肾中药龟板抗Parkinson病大鼠模型黑质神经元凋亡的作用,本研究用6-羟基多巴胺(6-OHDA,0.2%)于大鼠左侧黑质致密带注射2μl造成Parkinson病(PD)模型,同时设立龟板组(灌胃补肾中药龟板2g/次,2次/d,连续12周)、模型组和正常对照组,观察其行为改变,再用HE、TH染色或DIG-dUTP染色观察黑质神经元的变化,免疫组化染色检测Bcl-2和Bax蛋白的表达。结果显示:龟板组PD大鼠的旋转行为改善明显,黑质致密部的TH染色阳性神经元增多,DIG-dUTP染色阳性率降低,Bcl-2蛋白表达增加和Bax蛋白表达减少。本文结果提示,长期龟板治疗对PD模型大鼠多巴胺能神经元凋亡具有明显的保护作用,且该作用可能与龟板升高Bcl-2和降低Bax的表达有关。To study the anti-apoptosis effect of Plastrum Testudinis （PT） on the substantia nigra neurons of rats with Parkinson＇s disease （PD）, 6-hydroxydapamine （0. 2% , 2 μl） was injected into the rats＇compact zone of substantia nigra of the left side to induce PD. All animals were randomly divided into three groups： PT treatment group, model control group and normal control group. The rats in PT treatment group were treated by intragastric administration （4 g/d, twice one day） for 12 weeks. The behavior changes of rats were observed, the changes of substantia nigra neurons of rats were assayed by HE staining, tyrosine hydroxylase （TH） staining and digoxigenin deoxyuridine triphosphate （DIG-dUTP） staining, and the expression of Bcl-2 and Bax proteins was determined by immunohistochemistry. The results showed that the rotational behavior of PD rats in PT treatment group was ameliorated significantly. In the compact zone of substantia nigra, the TH immunopositive neurons increased and DIG-dUTP stained neurons decreased. On the other hand, the expression of Bcl-2 increased and the expression of Bax decreased. The results suggest that long-term treatment with PT can prevent the apoptosis of substantia nigra neurons in rats with Parkinson＇s disease and this effect maybe related PT with the increased Bcl-2 and decreased Bax expression.

关 键 词：龟板 PARKINSON病 多巴胺能神经元 凋亡 大鼠 

分 类 号：R285.5[医药卫生—中药学]