利用纤维床反应器固定化发酵生产丙酸  

Propionic Acid Fermentation by Propionibacterium freudenreichii CCTCC M207015 with a Fibrous-bed Bioreactor

作　　者：冯小海[1] 吴波[1] 沈晓波[1] 徐虹[1] 

机构地区：[1]南京工业大学制药与生命科学学院材料化学国家重点实验室,南京210009

出　　处：《生物工程学报》2008年第6期1075-1079,共5页Chinese Journal of Biotechnology

基　　金：国家"973"项目(2007CB714304);国家"863"项目(No.2006AA03Z453)资助~~

摘　　要：构建了一种纤维床反应器(FBB),并将其应用于丙酸的生产。将棉纤维绕成桶状,固定于反应器中,即可用于丙酸固定化发酵。以40g/L的葡萄糖为碳源,与游离细胞相比,利用FBB生产丙酸,丙酸产量由14.58g/L提高至20.41g/L,发酵时间由120h缩短至60h。研究了不同糖浓度条件下FBB生产丙酸情况,并将补料策略应用于丙酸发酵中。结果表明:补料发酵能够有效改善Propionibacterium freudenreichii CCTCC M207015在高糖条件下丙酸对葡萄糖转化率较低、副产物较多的问题。经补料发酵280h,丙酸产量达45.91g/L,丙酸质量约占有机酸总质量比例为72.31%。The production of propionic acid by Propionibacterium freudenreichii CCTCC M207015 was investigated in a Fibrous-bed bioreactor （FBB）. The FBB was constructed by packing spiral cotton fibrous and immobilized into a bioreactor. By applying this bioreactor to propionic acid fermentation, the propionic acid yield had a significant improvement and reached 20.41 g/L, compared with the cell-free culture of 14.58 g/L （40 g/L of glucose）. At the same time, the glucose exhausting time decreased from 120 h to 60 h. Batch fermentations at various glucose concentrations were carded out with FBB. Based on the analysis of the time course of production, fed-batch fermentation was also applied to produce propionic acid with FBB, the maximal propionic acid yield reached 45.91 g/L, and the proportion of propionic acid to total acids was about 72.31%.

关 键 词：纤维床反应器 PROPIONIBACTERIUM freudenreichii CCTCC M207015 丙酸 补料发酵 

分 类 号：TQ225.123[化学工程—有机化工]