不同动物源性大肠杆菌多重耐药调控基因SdiA的同源性分析  

Homology analysis of multiple drug resistance gene SdiA of Escherichia coli from different animal species

作　　者：马红霞[1] 丛薇[1] 刘玉堂[1] 伞治豪[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118

出　　处：《中国预防兽医学报》2008年第6期435-439,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(30400326);吉林省杰出青年基金(20050124);吉林省科技厅项目(20030425-03);吉林农业大学博士后启动基金项目资助

摘　　要：为研究大肠杆菌多重耐调控基因SdiA与不同动物源性及多重耐药水平之间的关系,选取临床分离的不同动物源性大肠杆菌5株及大肠杆菌药敏质控株ATCC25922,在对其进行主要治疗药物耐药性检测的基础上,分别以其染色体DNA为模板,通过PCR反应扩增出大肠杆菌多重耐药调控基因SdiA,将该片段分别与克隆载体pMD18-T连接,连接产物转化至大肠杆菌JM109感受态细胞中,对经酶切与PCR反应鉴定为阳性的克隆质粒进行插入片段的核苷酸序列测定。测序结果表明不同动物源性大肠杆菌的SdiA基因的核苷酸序列及所推导的氨基酸序列与GenBank中该基因序列的同源性较高,不同源性大肠杆菌的SdiA基因的核苷酸序列及氨基酸序列变化与其动物源性无关,该基因的核苷酸序列及氨基酸序列的个别突变位点可能影响AcrAB-TolC外排泵的表达水平,进而影响其多重耐药水平。To study the relationship between multi-drug resistant gene SdiA of different animal source and level of multi-drug resistance, The SdiA genes of 5 Escherichia coil strains and control strain ATCC25922 were amplified by polymerase chain reaction from chromosome and sequenced. The SdiA genes of Escherichia coli isolated from different animal species shared high homology at nucleotides level and deduced amino acids sequence level. Mutations of the genes were not related to animal species. However some mutations may be related to the level of drug resistance by influencing on the expression level of AcrAB-TolC efflux pump.

关 键 词：大肠杆菌 多重耐药 SdiA 克隆 同源性分析 

分 类 号：S852.6[农业科学—基础兽医学]