ESAT-6及PPDELISA检测疫区和非疫区牛结核病的比较研究  

Detection of bovine tuberculosis by ESAT-6-ELISA and PPD-ELISA:A comparative study

作　　者：张喜悦[1] 姜秀云[1] 宁浩然[1] 赵昆[2] 范伟兴[2] 吴延功[2] 王志亮[2] 何昭阳[1] 

机构地区：[1]吉林农业大学动物科技学院,吉林长春130118 [2]中国动物卫生与流行病学中心,山东青岛266032

出　　处：《中国预防兽医学报》2008年第6期455-459,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家863计划项目(2007AA022405)

摘　　要：本实验克隆、表达了牛结核早期分泌靶抗原蛋白6(ESAT-6)抗原,建立了ESAT-6酶联免疫吸附检测方法(ELISA)。同时应用牛结核提纯菌素(PPD)ELISA和ESAT-6-ELISA对采自疫区的641头牛和非疫区的324头牛进行检测,其中PPD-ELISA检测中,疫区有581头阳性牛,阳性率为90.64%,非疫区有2头阳性牛,阳性率为0.62%;ESAT-6-ELISA检测中,疫区有567头阳性牛,阳性率为88.45%,非疫区没有阳性牛。对采自疫区的23例变态反应阳性牛和非疫区的7例变态反应阳性牛进行检测,其中PPD-ELISA检测中,疫区有20头为阳性,与变态反应的符合率为86.90%,非疫区有1头为阳性,与变态反应的符合率为14%;在ESAT-6-ELISA检测中,疫区有18头为阳性,与变态反应的符合率为78.30%,非疫区没有阳性。这些结果表明:ESAT-6-ELISA的敏感性要低于PPD-ELISA;牛结核ELISA在非疫区应用效果较差,在疫区更具有应用价值。ESAT-6 （Early secretory antigenic target-6） ELISA was developed using a recombinant ESAT-6 antigen, The assay was compared with bovine purified protein derivative ELISA （PPD-ELISA） by testing against 641 bovine sera collected from epidemic area and 324 bovine sera from nonepidemic area. The PPD-ELISA detected 581 positive sera with a positive rate of 90.64 % in epidemic area, and 2 positive sera at a positive rate of 0.62 % in non-pidemic area, In comparison, 567 sera were detected positive by ESAT-6 at a positive rate of 88.45 % in epidemic area, no positive sera can be detected in non-epidemic area. The positive rate of both assays were highly consistent with shin test results. The ESAT-6-ELISA appeared less sensitive than PPD-ELISA. It was suggested that the bovine tuberculosis ELISA would be more useful in epidemic area than other area.

关 键 词：牛结核 ELISA PPD ESAT-6 

分 类 号：S852.618[农业科学—基础兽医学]