三肽囊素抗独特型ScFv噬菌体展示文库的构建  

Construction of a ScFv phage display library of the Bursin anti-idiotype antibody

作　　者：邬向东[1] 谌南辉[1] 李麟[1] 何后军[1] 袁汉英[2] 王萍[1] 

机构地区：[1]江西农业大学动物科学技术学院,江西南昌330045 [2]复旦大学遗传研究所,上海200433

出　　处：《中国预防兽医学报》2008年第6期482-485,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：江西省自然科学基金项目(0130023);国家自然科学基金项目(30560107)

摘　　要：利用在体外构建的三肽囊素抗独特型抗体可变区VH和VL基因,通过重叠延伸反应(SOE),以(Gly4Ser)3为连接肽,将VH和VL基因连接成为VH-Linker-VLScFv,且ScFvDNA与噬菌粒载体pHEN1的连接产物转化于大肠杆菌TG1,经辅助噬菌体M13K07感染后,获得重组的鸡源抗三肽囊素抗独特型抗体全套单链噬菌体抗体库,并将该抗体库展示在噬菌体表面,以利用噬菌体展示技术的强大筛选能力筛选出与三肽囊素同功能单链抗体(Bursin-ScFv)。The repertoire of VH and VL gene fragments was amplified from cDNA templates. ScFv was constructed by splicing by over.lap extension reaction using （Gly4Ser）3 as a linker. The ScFV DNA was inserted into phagemid vector （pHENI） and transfected into E, coli TGI that was infected with helper phage MI3K07. A ScFv phage display library of the Bursin anti-idiotype antibody was successfully constructed.

关 键 词：三肽囊素 抗独特型抗体 可变区重链和轻链基因 单链抗体 噬菌体抗体库 

分 类 号：S852.43[农业科学—基础兽医学]