急性呼吸道疫情病原学的快速鉴别  

ETIOLOGIC FAST IDENTIFICATION TO ACUT RESPIRATORY TRACT INFECTION

作　　者：余鹏博[1] 庄贵华[1] 王丽[2] 马萍[2] 田小平[2] 崔萌[2] 王敬军[2] 张立实[3] 

机构地区：[1]西安交通大学公共卫生学院流行病学与卫生统计学教研室,西安710061 [2]陕西省疾病预防控制中心 [3]四川大学华西公共卫生学院

出　　处：《现代预防医学》2008年第11期2147-2148,2151,共3页Modern Preventive Medicine

摘　　要：[目的]对2007年4月起在陕西高陵县某小学和幼儿园暴发的群体性不明原因发热、咳嗽的急性呼吸道疫情进行病原学快速鉴别研究。[方法]采集流行区新发患儿13例的咽拭子标本,新鲜抽提核酸,对常见12种呼吸道RNA病毒进行反转录-聚合酶链反应(RT-PCR)和巢式PCR,阳性分离标本接种敏感细胞培养,病变细胞进行免疫荧光鉴定。[结果]13例标本中RT-PCR和巢式PCR检出流感病毒B型阳性7例,其他病毒的特异性条带未检出;全部标本接种MDCK狗肾细胞3d后仅7例阳性检出,流感病毒B型者均出现细胞病变效应,其余未见明显细胞病变;病变细胞对B型流感单抗的直接免疫荧光试验均为阳性反应。[结论]本次急性呼吸道疫情的病原体为B型流感病毒。[Objective] Etiologic study were conducted on primary school students with unknown mason acute respiratory disease that took place at in April 2007. [Methods] 13 cases were collected and RNA virus isolation was used in multiplex RT-PCR and nest-PCR assays that aimed at 12 respiratory passage RNA virus. Positive samples was inoculated sensitive cells, then identified the pathological cells by immunofluorescent test. [Results] Among 13 patients, 7 throat swab samples were detected out influenza B virus positive, specific straps of other virus were not detected from 13 samples. 7 positive samples made MDCK cells cytopathic effect, and immunofluorescent assay on pathological cells showed positive result. [ Conclusion] Pathogen of this acute respiratory diseases （ARD） outbreak in Gaoling County of Shanxi Province is influenza B virus.

关 键 词：急性呼吸道疫情 病原学检测 B型流感病毒 

分 类 号：R447[医药卫生—诊断学]