TLCS法测定排氚片中黄芪甲苷的含量  

Determination of Astragaloside Ⅳ in Paichuan Tablets by TLC-Scanning

作　　者：辛艳茹[1] 刘丽宏 马萍 杜树山[2] 张英娜 张智杰 李志韧 

机构地区：[1]解放军第二炮兵总医院药剂科,北京100088 [2]北京师范大学资源学院教育部资源药物工程研究中心,北京100088 [3]不详

出　　处：《解放军药学学报》2008年第3期258-260,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：全军十一五重点课题;No.200603004

摘　　要：目的建立测定排氚片中黄芪甲苷含量方法。方法采用薄层扫描法测定黄芪甲苷含量，硅胶G薄层预制板，氯仿-甲醇-水（13：6：2）10℃以下放置过夜的下层溶液为展开剂，测定波长530nm，参比波长700nm。结果黄芪甲苷点样量在0．20～0．701μg之间和吸收度积分值呈良好的线性关系，平均回收率为96．74％，RSD为2．02％。结论薄层扫描法便捷、灵敏、准确，可作为排氚片的质量控制方法。Aim To develop a method for determining the content of astragaloside IV in paichuan tablets by TLC - scanning. Methods Silica G was used as the solid phase and developed with Chloroform-Methanol-Water （ 13： 6：2）; λS = 530nm, λR = 700nm. Results A good linearity of astragaloside IV was obtained within the range of 0.20-0. 70μg. The average recovery was 96.74% and RSD was 2.02%. Conclusion The method was simple, accurate, reliable and can be used as the quality control of this preparation.

关 键 词：排氚片 黄芪甲苷 TLCS 含量测定 

分 类 号：R927.2[医药卫生—药学]