HPLC法对三产地天麻中天麻素的含量测定  

Improvement of Determination of Gastrodin in Gastrodia Elata Blume. by HPLC

作　　者：田野[1] 李秀芳[1] 杨莲[1] 陈顺芳 周宁娜[1] 林青[1] 

机构地区：[1]云南中医学院,云南昆明650200 [2]昭通市天麻研究所,云南昭通657000

出　　处：《云南中医学院学报》2008年第3期6-8,共3页Journal of Yunnan University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目。(NO:30760305。)

摘　　要：目的：测定三个不同产地天麻中天麻素含量。方法：采用HPLC法测定三产地天麻中天麻素的含量。Agilent TC—C18色谱柱（4．6mm×250mm，5μm）；流动相：乙腈-0．05％磷酸（4：96）；流速：1．0mL／min；检测波长：220nm。结果：天麻素在20～200μg的范围内呈现良好的线性关系，平均回收率为97．93％，RSD为1．49％（n=9）。云南昭通、湖北宜昌、陕西西乡三产地天麻中天麻素质量百分含量分别为0．164％，0．263％，0．418％．结论：三产地的天麻中天麻素含量有明显差异，云南昭通产天麻中天麻素含量最低。Objective： To determine the content of gastrodin in three different habitats/Gastrodia elata Blume. Method： To determine the content of gastrodin in three different habitats/ Gastrodia elata Blume by HPLC, on Agilent TC- C18 column, with Methylcyanide- 0. 05% phosphoric acid （4：96） as mobile phase, 1.0mL/ min as speed of flow, detection wavelength at 220nm. Result ： The good linear correlation was obtained in the range 20 - 200μg. The average recovery of spots （ n = 9） was 97.93 % （ RSD = 1.49 % ）. The content of gastrodin in the Yunnan Zhaotong, the Hubei Yichang, the Shanxi Xi Town respectively was 0. 164%, 0. 263%, 0. 418%. Conclusion： There were obvious differences in the content of gastrodin in three different habitats＇ Gastrodia elata Blume. The content of gastrodin in Yunan Zhaotong＇s Gastrodia elata Blume was the lowest.

关 键 词：HPLC法 天麻素 含量测定 

分 类 号：R284.1[医药卫生—中药学]