2型糖尿病患者外周血单个核细胞过氧化物酶体脂肪酸β氧化活性变化的研究  

Study on the activities of peroxisome β-oxidation of peripheral blood mononuclear cell from T2DM patient

作　　者：李继红[1] 李蓟龙[2] 杨志东[3] 罗强[1] 赵铁军[1] 贾天军[1] 魏会平[1] 刘继云[1] 姜玲玲[4] 

机构地区：[1]河北北方学院基础医学部生物教研室,张家口075000 [2]河北北方学院学报 [3]河北北方学院信息管理系,张家口075000 [4]河北医科大学生物化学与分子生物学教研室,石家庄

出　　处：《中国糖尿病杂志》2008年第5期291-293,共3页Chinese Journal of Diabetes

基　　金：河北省科学技术研究与发展指导计划基金资助项目(062761965)

摘　　要：目的探讨2型糖尿病(T2DM)患者脂代谢紊乱状态下过氧化物酶体脂肪酸β氧化活性的变化。方法分析112例T2DM患者外周血单个核细胞过氧化物酶体脂肪酸β氧化活性、脂肪乙酰辅酶A氧化酶活性及其mRNA表达变化。测定FPG、TC、TG、HDL-C、LDL-C。结果T2DM患者过氧化物酶体脂肪酸β氧化代偿性加强,与正常对照组相比,脂肪乙酰辅酶A氧化酶活性、过氧化物酶体脂肪酸β氧化活性增加;并且T2DM组过氧化物酶体脂肪酸β氧化的活性与其血清TG、HDL-C、LDL-C之间有直线相关关系。结论脂肪酸β氧化和脂肪乙酰辅酶A氧化酶的活性增加,导致H2O2浓度的升高,这可能是糖尿病心血管并发症发病的原因之一。Objective To explore the change of the activities of peroxisome β-oxidation in T2DM patients with the lipid disorders. Methods We analyzed the changes of the activities of peroxisome oxidation and fatty acyl-CoA oxidase and its mRNA expression in 112 cases of T2DM patients. Results We found that there was compensatory increase in activity of peroxisome β-oxidation of T2DM patients. As compared with control group, the activity of fatty acyl-CoA oxidase of T2DM group was increased by 5% （P〈0.05）, the activity of peroxisome β-oxidation was increased by 37%（P〈0. 05）, There were linear correlations of theof peroxisoraal β-oxidation with serum TG, HDL, and LDL. Conclusions The increase of the activities of peroxisomal β-oxidation and fatty acyLCoA oxidase leads the increase of H2 02 concentration. This may be one of the causes of T2DM cardiovascular complications.

关 键 词：糖尿病 2型 过氧化物酶体 脂肪酸β氧化 酯酰辅酶A氧化酶 

分 类 号：R587.1[医药卫生—内分泌]