高糖和SB203580对大鼠肾系膜细胞色素上皮细胞衍生因子表达的影响  

Effect of high glucose and SB203580 on the expression of PEDF in cultured glomerular mesangial cells

作　　者：钟海花[1] 徐勇[1] 顾峻菱[1] 万沁[1] 

机构地区：[1]泸州医学院附属医院内分泌科,四川646000

出　　处：《中国糖尿病杂志》2008年第5期307-309,共3页Chinese Journal of Diabetes

摘　　要：目的探究高糖以及SB203580对大鼠肾系膜细胞的色素上皮细胞衍生因子(PEDF)表达的影响。方法将大鼠HBZY-1肾小球系膜细胞分为7组,将高糖作为刺激因子,SB203580作为干预因素。分别设正常对照组、高糖组(包括3种浓度的葡萄糖)、甘露醇组、治疗组以及溶剂对照组。用RealtimeQuantitativePCR法测定各组系膜细胞PEDFmRNA表达。结果(1)正常组系膜细胞PEDF在mRNA的水平上有表达,高糖组系膜细胞PEDFmRNA的表达较正常对照组明显降低。(2)治疗组的系膜细胞PEDFmRNA表达较高糖组明显增加。结论高糖可抑制大鼠肾系膜细胞PEDF的表达,并与P38MAPK信号通路有关。Objective To explore the effect of high glucose and SB203580 on the expression of pigment epithelium-derived factor （PEDF） in cultured glomerular mesangial ceils （GMC）. Methods Cultured HBZY-1 rat GMC were divided into 7 groups（glucose was used as a stimulating factor, SB203580 as an intervenor） ： normol glucose （5.6retool/L） group, high glucose-treated group （10,20,30retool/L）, mannitol-treated group, therapy （SB203580） group, drug-solvent control group. We measured the expression of PEDF of each glomerular mesangial cells group by real time quantitative PCR. Results 1. Normol glucose group showed the higher expression of PEDF than did the high glucose group. The expression of PEDF mRNA of group of normol glucose was 5.3, 11.3, 27.9 times elevated above high glucose groups （all P〈0. O5 ）. 2. The expression of PEDF mRNA was higher in therapy group than in high glucose group. The expression of PEDF mRNA in therapy group was 31.8 times that of high glucose group （P〈O. 05 ）. Conclusions High glucosc can decrease significantly the expression of PEDF in cultured mesangial ceils, which is independent of the osmotic pressure. Downregulated expression of PEDF induced by high glucose is related with P38MAPK signal transduction pathways.

关 键 词：糖尿病肾病 高糖 肾小球系膜细胞 P38MAPK 色素上皮细胞衍生因子 

分 类 号：R587.2[医药卫生—内分泌]