锰胁迫对商陆(Phytolacca acinosa)保护酶的影响  

Effects of protective enzymes of Phytolacca acinos under Mn anganese stress

作　　者：陈燕珍[1] 陶毅明[1] 梁振鑫[1] 梁杨琳[1] 刘晓灿[1] 

机构地区：[1]广西师范大学生命科学学院,桂林541004

出　　处：《生物学杂志》2008年第3期44-47,共4页Journal of Biology

基　　金：广西博士学位授权点学科建设专项经费资助项目(XKY2006ZD02)

摘　　要：对室内栽培的商陆(Phytolacca acinosa)幼苗分别经4、12、36 mmol/L的锰(Mn2+)溶液处理30d后,用聚丙烯酰胺凝胶电泳(PAGE)分离了根和叶片过氧化物酶(POD)同工酶和超氧化物歧化酶(SOD)同工酶,并测定了其酶活性。结果表明:(1)经不同浓度的Mn2+溶液处理,根POD同工酶(RP1)消失,而12、36 mmol/L Mn2+溶液处理根新的POD同工酶(RP5,RP8)出现,根POD酶活性出现先降低后升高的变化趋势;叶片POD同工酶(LP)有3种,与对照的相同,但其酶活性随Mn2+浓度增加而随之升高。(2)12、36 mmol/L Mn2+溶液处理,根新的SOD同工酶(RS5-RS7)出现,SOD酶活性上升;36 mmol/L Mn2+溶液处理,叶片SOD同工酶(LS2-LS5)消失,叶片SOD酶活性下降。推断,商陆根和叶片同工酶的表达调控的机制不同。认为,商陆在Mn2+胁迫中,POD发挥更重要的作用。The Phytolacca acinosa seedlings were treated with different Mn2＋ oncentrations of 4, 12 and 36mmol/L for 30 days at greenhouse. The isoforms of POD and SOD in roots and leaves were separated by means of PAGE and their enzyme activities were determined. The results were as follows ： （ 1 ） POD isoenzyme in root （ RP1 ） disappeared when different concentrations of Mn2＋ were applied, while additional POD isoenzymes （RP5 and RP8） appeared when 12 and 36mmol/L Mn2＋ were applied. The POD activities in root first increased and then decreased but the POD activities in leaves decreased as the concentration of Mn2＋ was enhanced. 3 POD isoenzymes were observed in leaves. （2） When 12 and 36 mmol/L Mn2＋ were applied, SOD activi- ties in roots increased compared with the control and additional SOD isoenzyme RS5- RS7 were observed. When 36 mmol/ L Mn2＋ was treated, the SOD isoenzymes in leaf LS2-LS5 disappeared accompanied with the decline of SOD activities. It was suggested there were different regulation mechanisms in the expression of isoenzyme in root and leaf of Phytolacca acinosa. It is indicated that POD plays a more important role when the Phytolacca acinosa is under Mn stress.

关 键 词：锰 商陆 胁迫 过氧化物歧化酶 超氧化物歧化酶 

分 类 号：Q945.78[生物学—植物学]