SNPlex系统检测方法及在法医遗传学中的应用前景  

Test methods and the application of SNPIex genotyping system in forensic science

作　　者：于子辉[1] 李彩霞[2] 季安全[2] 孙辉[2] 苏芹[1] 严红[2] 胡兰[2] 

机构地区：[1]中国人民公安大学,法医遗传学北京100038 [2]公安部物证鉴定中心,北京100038

出　　处：《刑事技术》2008年第3期41-45,共5页Forensic Science and Technology

基　　金："十一五"国家支撑计划项目(2006BAK07B01)

摘　　要：被称为第三代遗传标记的SNP是近年来的研究热点,对SNP检验检测方法很多,例如引物延伸结合时间飞行质谱分析法,微矩阵基因分型法,SNPlex基因分型系统等,本文重点介绍的就是SNPlex基因分型系统,及其法医学应用前景。这种系统是利用DNA模板直接与等位基因特异探针和位点特异性探针结合,连接产物经扩增之后与特异探针杂交结合,最后通过毛细管电泳检测特异探针进行SNP分型。SNPs which is called the third generation of genetic markers are increasingly concerned nowadays. There are various kinds of SNP detection techniques, such as primer extention MOLDI-TOF, microarray, SNPlex system, etc. The SNPlex genotyping system and its prospect of forensic science application were introduced in this paper. In the SNPlex system the DNA template was detected directly by the allele specific probes. The ligation products were amplified, the specific probes were used to hybridize with the amplification product, and the SNP genotypes were determined by the capillary electrophoresis results of the specific probes.

关 键 词：法医遗传学 SNP SNPlex 应用前景 

分 类 号：D919[医药卫生—法医学]