呼吸道合胞病毒感染时间对花粉诱导的过敏性反应的影响  

Relationship Between Primary RSV Infection and JCP-induced Allergic Responses

作　　者：刘北星[1] 吕昌龙[1] 阎建忠[1] 那立新[1] 木村吉延[2] 

机构地区：[1]中国医科大学基础医学院免疫学教研室,沈阳110001 [2]福井大学医学部微生物学教研室,日本

出　　处：《中国医科大学学报》2008年第3期314-316,共3页Journal of China Medical University

基　　金：教育部留学回国人员启动基金资助项目(教外司留2004527)

摘　　要：目的探讨呼吸道合胞病毒(RSV)感染时间对花粉诱导的呼吸道过敏性反应是否有影响作用。方法用花粉抽提物JCP致敏BALB/c鼠,致敏前或后经鼻感染RSV,提取肺组织细胞,体外培养,ELISA试剂盒检测培养上清中细胞因子水平;姬姆萨染色法分类并计数肺组织内浸润细胞数量;ELISA方法检测血清特异性抗体IgE水平。结果JCP致敏鼠肺组织细胞培养上清中Th2型细胞因子IL-4明显升高,血清JCP特异性IgE抗体水平亦显著上升,提示JCP是Th2型免疫应答的诱导剂。致敏前RSV感染诱导BALB/c鼠肺组织细胞合成分泌Th1型细胞因子,下调JCP诱导的Th2型免疫应答,降低致敏鼠肺组织炎性细胞的浸润,减少前炎性因子TNF-α及趋化因子IL-8的分泌,降低血清JCP特异性IgE抗体水平。结论在某种条件下,RSV感染对花粉过敏原诱导的过敏性反应有一定的保护作用。Objective To explore the effect of the timing of respiratory syncytial vires infection on JCP-induced respiratory allergy in mice. Methods BALB/c mice were intranasally infected with respiratory syncytal virus （t/SV） before or after sensitization to Japanese cedar pollen crude extract （JCP）. Single-cell suspensions obtained from the lung parenchyma of inoculated mice were re-stimulated in vitro by cocultivating with UV-RSV or JCP for 48 hr. Production of cytokines in the culture fluid of lung parenchyma cells and the level of antigen-specific IgE in the serum were determined. Identification of lung inflammatory cells was performed by using Giemsa-staining. Results The level of intedeukin （IL）-4 in the hmg tissues and JCP-specific IgE in serum were increased significantly by JCP sensitization,suggesting that JCP was a stronger inducer for Th2-type allergic responses. RSV infection, before JCP-sensitization, decreased JCP-induced ＇Ila2 type responses and the production of TNF-α and IL-8 in lung tissues and lung inflammatory cells through anlFN-3, response. Conclusion Under a cerlain condition, RSV infection may play a protective role in JCP-induced allergic responses.

关 键 词：呼吸道合胞病毒 花粉抽提物 TH1/TH2型细胞因子 

分 类 号：R392.11[医药卫生—免疫学]