丙酮酸乙酯对急性坏死性胰腺炎大鼠肠黏膜的保护作用  

Role of Ethyl Pyruvate Against Intestinal Mucosal Barrier Dysfunction during Murine Acute Necrotizing Pancreatitis

作　　者：栾正刚[1] 张成[2] 董明[2] 马晓春[1] 郭仁宣[2] 

机构地区：[1]中国医科大学附属第一医院重症监护室,沈阳110001 [2]中国医科大学附属第一医院普外科,沈阳110001

出　　处：《中国医科大学学报》2008年第3期340-342,共3页Journal of China Medical University

摘　　要：目的探讨丙酮酸乙酯(EP)减轻急性坏死性胰腺炎(ANP)肠黏膜损伤及其治疗肠屏障功能障碍的机制。方法逆行胰胆管注射5%牛磺胆酸钠制作ANP模型。雄性Wistar大鼠随机分成3组,假手术组、ANP组和EP治疗组。测定血浆D-乳酸、肠组织髓过氧化物酶(MPO)水平,半定量逆转录聚合酶链反应(RT-PCR)法检测肠组织高迁移率族蛋白B1(HMGB1)mRNA表达,并观察肠组织形态学改变。结果ANP组血浆D-乳酸、肠组织MPO在建模后24h达高峰,48h仍维持在较高水平,EP治疗组血浆D-乳酸、肠组织MPO水平明显低于ANP组(P<0.05)。ANP组大鼠肠组织HMGB1mRNA表达水平在ANP后12h明显升高,至24h达峰,48h仍维持在高水平。EP治疗组肠组织HMGB1mRNA表达水平均明显低于ANP组(P<0.05)。结论EP能显著下调血浆D-乳酸、肠组织MPO水平并明显抑制HMGB1基因表达,改善肠黏膜屏障功能,对ANP肠黏膜损伤有明显保护作用。Objective To investigate the effects of ethyl pyruvate （EP） on the levels of D-lactate in plasma and the activity of myeloperoxidase （ MPO ） in the intestinal tissues and mRNA expression of high mobility group box 1 （ HMCB1 ） in intestinal mucosal tissues, and to explore the mechanisms of ethyl pyruvate in protecting the intestinal mucosal barrier against injury induced by acute necrotizing pancreatitis （ANP）. Methods ANP model was established by retrograde injection of 5% sodium tauroeholate into panereatie duct. Animals were divided randomly into three groups：eontrol group, ANP group, and EP treatment group. EP solution was administered intravenously every 6 hours （40 mg/kg once）. The mRNA expression of HMGB1 in bowel tissues was detected by reverse transcription-polymerase chain reaction （RT- PCR ）. The histological examination of the bowel was also performed. Results The levels of D-lactate and MPO were rapidly increased after ANP model was established, and reached the peak at the 24th hour and remain relatively high up to the 48th hour compared with the control group. In comparison with those in ANP group, the levels of D-lactate and MPO were markedly lowered in EP group 24 and 48 houIs after ANP model was set up （P 〈 0.05 ）. The mRNA expression of HMGB1 in bowel tissues was increased significantly at the 12th hour and maintained to the 24th hour after ANP model was set up, whereas in EP group, HMGB1 mRNA expression was significantly lower than that in ANP group at each time point （ P 〈 0.05 ）. The injury of bowel tissues in EP group was milder than that in ANP group. Conclusion EP can decrease the levels of D-lactate and MPO,down-regnlate HMGB1 mRNA expression in intestinal tissues of ANP rats,and protect intestine from injury induced by ANP.

关 键 词：高迁移率族蛋白B1 急性坏死性胰腺炎 肠黏膜屏障 丙酮酸乙酯 

分 类 号：R657.5[医药卫生—外科学]