碳纳米管组装电化学免疫传感器测定IgG抗体的研究  

Amperometric Immunosensor for Anti-IgG Antibody Based on a Glassy Carbon Electrode Modified with Multiwall Carbon Nanotubes

作　　者：张东东[1] 漆红兰[1] 李小蓉[2] 

机构地区：[1]陕西省生命分析化学重点实验室,陕西师范大学化学与材料科学学院,西安710062 [2]陕西中医学院药学系,陕西咸阳712046

出　　处：《传感技术学报》2008年第5期719-723,共5页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助National Science Foundations of China(90607016)

摘　　要：应用吸附法将IgG抗原固定于多壁碳纳米管修饰的玻碳电极表面,制备用于IgG抗体检测的电化学免疫传感器。以辣根过氧化物酶为标记物,对苯二酚为底物,利用辣根过氧化物酶标记IgG抗体与待测IgG抗体竞争电极表面固定的IgG抗原,建立了免疫竞争法检测IgG抗体的高灵敏度电化学分析方法。碳纳米管的大比表面积和电化学催化作用,提高了分子识别物质的固定量和电化学检测的灵敏度。工作电位为＋0.030 V（vs.SCE）时,响应电流与IgG抗体浓度在0.30-10μg/mL范围内呈良好的线性关系,检出限为0.11μg/mL。A highly sensitive amperometric immunosensor for anti-IgG antibody based on a glassy carbon electrode modified with multiwall carbon nanotubes （MWNT） was developed. A MWNT layer formed on a glass carbon electrode was utilized as a sensing platform for the immobilization of IgG antigen and a competitive immunoreaction occurred while analyte anti-IgG antibody and horseradish peroxidase （HRP）-labeled anti-IgG antibody competed for the surface-immobilized IgG antigen binding sites. The catalytic reduction current at t0. 030 V （vs. SCE）, which was produced in the reaction of hydroquinone with hydrogen peroxide in the presence of HRP, was linear over a range from 0. 30 to 10 μg/mL anti-IgG antibody with a detection limit of 0. 11 μg/mL.

关 键 词：电化学免疫 碳纳米管 IGG 辣根过氧化物酶 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]