骨组织免疫组织化学染色特点及其质量控制  

Immunohistochemistry Staining of Bone Tissues and Quality Control

作　　者：袁永辉[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院病理研究所,湖北武汉430030

出　　处：《实验室研究与探索》2008年第4期21-23,共3页Research and Exploration In Laboratory

摘　　要：为了建立骨组织的制片与免疫组织化学染色的标准化实验方法,达到免疫组织化学检测的质控要求,详细介绍了骨组织的取材、固定、脱钙、切片、免疫组织化学和TUNEL染色及其质量控制环节。用此法制备的免疫组织化学切片具有阳性结果呈颗粒状的棕黄色,定位准确,对比明显,背景浅,组织结构与细胞轮廓完整、清晰,适合显微照相以及染色结果稳定可靠,特异性强、敏感性高。该方法可作为骨组织的制片与免疫组织化学的标准化实验方法应用。In order to establish a standard experimental method for making paraffin-embedded sections and staining of immunohistochemlstry in bone tissues to meet the quality control demand in immunohistochemistry tests, the procedures for sampling, formalln-fixation, decalcification, dehydration, clearing, paraffin embedding, cutting into sections and staining of immunohlstochemistry and terminal-deoxynucleotidyl transferase mediated x-dUTP nick end labeling （TUNEL） of bone tissues were described. The sections by this method can show special yellow granule in positive cells; they reveal the more accurate location, markedly characteristic contrast, weaker background with good visibility, more detail and clear structure of tissues and cells, and are suitable for microphotography. Also staining results by this method are stable and reliable with strong specificity and high sensitivity： This method can be applied as a standard experimental method to make paraffin-embedded sections and staining of immunohistochemistry and TUNEL in bone tissues

关 键 词：骨组织 免疫组织化学 TUNEL染色法 质量控制 

分 类 号：R322.71[医药卫生—人体解剖和组织胚胎学]