三氧化二砷对大鼠生精细胞端粒酶逆转录酶活性的影响  

The Effects of As_2O_3 on Telomerase Activity in Rat Spermatogenic Cells

作　　者：陈伟[1] 陆祥[1] 舒小林[1] 李季蓉[1] 

机构地区：[1]遵义医学院组织学与胚胎学教研室,贵州遵义563003

出　　处：《苏州大学学报（医学版）》2008年第2期188-190,F0002,共4页Suzhou University Journal of Medical Science

基　　金：贵州省国际科技合作重点资助项目(黔科合J字[2006]2078号)

摘　　要：目的观察不同剂量三氧化二砷(As2O3)对成年大鼠生精细胞端粒酶活性的影响。方法将40只健康雄性SD大鼠随机分成4组,分别为0.375、0.75、1.5mg/kg3个染毒组和对照组,采取灌胃法连续给药16周后对各组大鼠进行睾丸脏器系数、精子头计数,并计算每日精子生成量(DSP)。采用免疫组化法检测大鼠生精细胞端粒酶逆转录酶(TERT)活性的表达。结果(1)中剂量组(0.75mg/kg)和高剂量组(1.5mg/kg)睾丸精子头计数和DSP均明显低于对照组(P<0.05或<0.01);(2)与对照组相比,中、高剂量组大鼠生精细胞TERT阳性表达率显著降低(P<0.01);(3)生精细胞TERT活性与每日精子生成量成正相关(r=0.521,P<0.01)。结论一定剂量的As2O3可通过抑制生精细胞TERT的活性而导致精子生成量减少,产生生殖毒性。Objective To observe the expression of telomerase activity in spermatogenic cells after aniso-doses arsenic （As2O3） administration in the testes in the adult male rats. Methods Fourty healthy male Sprague-Dawle rats were divided into four groups randomly and they were administered respectively with 0（in the control group） and 0.375,0.75,1.5 mg/kg body weight of As2O3 by intragastric administration consecutively for 16 weeks. The numbers of testicular sperm head were counted and the coefficient of testicular viscera and daily sperm production（DSP） were calcu- lated in each group. The telomerase activity expression in spermatogenic cells in different grades were located and quantitated by method of immunohistochemistry. Results （1）In the testis of As2O3-treated rats, The coefficient of testicular viscera, the number of testicular sperm head and daily sperm production in middle and high doses group decreased significantly than that in the controls（P〈0.01）. （2）The expression of telomerase activity in the middle dose group and high dose group decreased significantly compared with that in the control group （P〈0.01）. （3）The dependability analysis between DSP and the expression of telomerase activity in spermatogenic cells showed a positive correlation （r=0.521,P〈0.01）. Conclusion One mechanism of male reproduction toxicity of As2O3 might be that telomerase activity is inhibited,which induces decrese of quantity of sperm cells.

关 键 词：AS203 端粒酶逆转录酶 生精细胞 大鼠 

分 类 号：R994.6[医药卫生—毒理学]