儿童下呼吸道感染的病毒病原学研究  

A STUDY ON UIRAS PATHOGENY OF THE INFANTILE LOWER RESPIRATORY TRACT INFECTION

作　　者：张朝明[1] 贺玉萍[1] 李智芳[1] 王军[1] 刘小凡[1] 

机构地区：[1]成都中医药大学附属医院

出　　处：《四川省卫生管理干部学院学报》1997年第4期210-211,共2页Journal of Sichuan Continuing Education College of Medical Sciences

摘　　要：目的：应用系列主要呼吸道病毒（呼吸道合胞病毒〈ＲＳＶ〉、腺病毒３．７型〈ＡｄＶ３．７〉、甲型流感病毒〈ＦｌｕＡ〉、乙型流感病毒〈ＦｌｕＢ〉、副流感病毒１．３型〈ＰＩＶ１．３〉、副流感病毒２型〈ＰＩＶ２〉共六种）单克隆抗体（ＭｃＡｂ），对１２３例下呼吸道感染（ＬＲＩ）患儿鼻咽部脱落细胞中的抗原进行检测，研究呼吸道病毒所致ＬＲＩ的流行情况。方法：碱性磷酸酶抗碱性磷酸酶（ＡＰＡＡＰ）桥联酶标法。结果：１２３例下呼吸道感染患儿中阳性病例７２例，阳性率５８．５％（７２／１２３），其中男为５８．９％（４３／７３），女为５８．０％（２９／５０），二者无显著性差异（Ｐ＞０．０５）；病毒检出率ＲＳＶ２３．６％、ＡｄＶ３．７１３．０％、ＦｌｕＡ４．９％、ＦｌｕＢ１０．６％、ＰＩＶ１．３７．３％，ＰＩＶ２３．３％。其中混合感染４．１％（５／１２３）。ＲＳＶ与其它五种病毒检出率有显著性差异（Ｐ＜０．０５），病原检出率在婴儿组、幼儿组与大于３岁儿童组之间无显著性差异（Ｐ＞０．０５）。结论：研究提示儿童ＬＲＩ一半以上与病毒感染相关。Objective:Applying monoclonal antibody(McAb) against the main and serial lower respiratory tract infection Viruses (RSV,ADV37,FLuA,FLuB,PIV13,PIV2),the antigens in the detached cells of nasopharyngeal region of LRI infants (n=123)were detected to study the epidemic condition of LRI caused by rirusMethods:The virus antigens were detected using engyme-linked McAb technique (McAb-APAAP)Results:Among 123 cases of infantile LRI 72 cases were positive (58.5%,72/123),of the 123 cases 58.9%(43/73) for males and 58.0%(29/50)for females,showing no significant difference (P>0.05);the rirus detecting rates:RSV 23.6%,ADV3.7 13.0%,FLuA4.9%,FluB106%,PIV1.3 7.3%and PIV2 3.3%,of which the mixed infection was 4.1%(5/123),There was significant differente for RSV detecting rate compared to that of the other viruses (P<0.05) and there wasn′t any significant differences between the new-borns,infants or children above 3 years old(P>0.05)Conclusion:This suggests that more than half of the infantile LRI cases are related to virus infection and RSV is the main virus resulting in LRI

关 键 词：呼吸道感染 单克隆抗体 病毒 病原学 儿童 

分 类 号：R725.6[医药卫生—儿科]