反相高效液相色谱法测定人血中血小板活化因子方法学的研究及其临床意义  

Study on measurement of plateletactivating factor in human plasma with reversed high performance liquid chromatography and its clinical significance

作　　者：王学谦[1,2,3,4] 吴胜群[1,2,3,4] 张雅敏[1,2,3,4] 胡文英 邬凤泉 徐新女[1,2,3,4] 

机构地区：[1]天津市第一中心医院急救医学研究所 [2]卫生部危重病急救医学重点实验室 [3]天津市南开大学医学院 [4]天津市第一医院

出　　处：《中国危重病急救医学》1997年第12期722-726,共5页Chinese Critical Care Medicine

基　　金：天津市卫生局科技基金

摘　　要：目的：探讨用反相高效液相色谱法（ＲＨＰＬＣ）测定人血中血小板活化因子（ＰＡＦ）方法学的实用性。方法：分析实验影响因素，并与生物法对比观察，对不同年龄段正常人与冠心病患者血中ＰＡＦ含量进行测定与分析。结果：证实ＲＨＰＬＣ法测定ＰＡＦ操作简便，与经典生物法有良好的相关性（ｒ＝０．９０７９）。结论：用ＲＨＰＬＣ法测定人血中ＰＡＦ的干扰因素少，重复性好，测定的血ＰＡＦ值具有可靠性，能较准确地反映血中ＰＡＦ的水平。Objective:To explore the practicality of measuring plateletactivating factor (PAF) with reversed high performance liquid chromatography (RHPLC).Methods:We analyzed the factors affecting PAF measurement by RHPLC method,and PAF was concomitantly determined for comparison by bioassay and RHPLC in healthy controls at different age range,and patients with coronary heart diseases.Results:The method of RHPLC was simple,convenient,and easy to operate.It had a good correlation with classical bioassay( r =0 907 9).Conclusions:The method of RHPLC has little interference and good duplication.Using this method,the level of plasma PAF we determined is reliable and accurate.

关 键 词：血小板活化因子 生物法 RHPLC 人血 

分 类 号：R446.112[医药卫生—诊断学]