透析器复用时IL-1β、TNF-α和IL-6基因表达及其血浆水平的研究  

The study of IL-1β,TNF-α and IL-6 gene expression and plasma levels on hemodialysis equipped with reused dialyzer

作　　者：钱家麒[1] 余志远[1] 戴慧莉[1] 黄佩文 张庆怡[1] 程砜 陈诗书[2] 

机构地区：[1]上海第二医科大学附属仁济医院肾脏科,200001 [2]上海第二医科大学附属仁济医院生化教研室分子生物学实验室,200001

出　　处：《中华肾脏病杂志》1997年第6期339-341,共3页Chinese Journal of Nephrology

基　　金：国家自然科学基金资助;上海市教委资助课题

摘　　要：目的 观察透析器复用对白介素-1β(IL-1β)、肿瘤坏死因子α(TNF-α)、白介素-6(IL-6)基因表达及其血浆水平的影响。方法 22例维持性血透患者随机分为铜仿膜(7例)、PMMA膜(7例)与聚砜膜(8例)组,采用ELISA、反转录多聚酶链反应及细胞原位杂交方法对细胞因子水平进行自身对照研究。结果 透析器复用后各细胞因子水平均有下降,但无显著差异;而其基因表达水平较初用有显著下降。结论 在一定的质量控制条件下,应用福尔马林消毒复用透析器可能会改善透析膜的生物相容性,这对减少透析费用及长期透析的有关并发症的发生有利。Objective To investigate the biocompan'bility of reused dialyzer. Methods Twenty-two maintained hemodialysis patients were randomly divided into cuprophan (7), PMMA (7) and polysulphone (8) groups by themselves control. IL-1β,TNF-α and IL-6 gene expression and plasma levels by ELISA>RT-PCR and in situ hybridization were observed during dialyzer reuse. Results Every plasma cytokine level was decreased during reuse compared with first use dialyzer, but no significant difference was found between them. The levels of gene expression of IL-1β、TNF-α and IL-6 were different from the first use significantly. Conclusion If effective dialysis volumn was maintained, formaldehyde as disinfectant on reprocessing the dialyzer may amilorate membrane bio-compatibility. It would be benificial to decrease appearance of long term hemodialysis -related complications.

关 键 词：肿瘤坏死因子 基因表达 透析器复用 IL-1 IL-6 

分 类 号：R459.5[医药卫生—治疗学]