父系遗传背景对小鼠超排效果及孤雌胚胎体外发育的影响  

Effects of paternal genetic background on efficiency of superovlaton and development of mouse parthenogenetic embryos in vitro

作　　者：严兴荣[1] 余树民[2] 雷安民[1] 沈文正[1] 黄伟伟[1] 贾文文[1] 窦忠英[1] 

机构地区：[1]西北农林科技大学动物医学院国家干细胞工程技术研究中心陕西分中心,陕西杨凌712100 [2]四川农业大学动物科技学院,四川雅安625000

出　　处：《西北农林科技大学学报（自然科学版）》2008年第6期1-4,共4页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家“863”计划项目(2005AA219050);教育部重大项目(03160)

摘　　要：【目的】研究父系遗传背景对小鼠超排效果及其孤雌胚胎体外发育的影响。【方法】以3种近交系雄鼠(129/Sv、C3H和C57BL/6J)与昆明系(KM)雌鼠的杂交一代(F1)及KM自交系小鼠为研究对象,对其进行超排处理,比较3种不同遗传背景的杂交小鼠与对照KM自交系小鼠的超排效果,分析其孤雌胚胎体外激活率和囊胚率的差异。【结果】3种F1代杂交小鼠与对照KM的超排效果和孤雌胚胎的激活率没有显著差异(P>0.05);129/Sv×KM、C3H×KM孤雌胚胎的囊胚率显著高于其他各组(P<0.05);C57BL/6J×KM与KM×KM之间孤雌胚胎囊胚发育率的差异不显著(P>0.05)。【结论】129/Sv、C3H和C57BL/6J父系遗传背景对杂交F1代小鼠的超数排卵数量和激活率没有影响,但引入129/Sv和C3H父系遗传背景可促进杂交F1代小鼠孤雌胚胎的体外发育,提高囊胚发育率。[Objiective] The paper studied the effects of paternal genetic background on the preimplantional development of mouse parthenogenetic embryos in vitro. [Method] F1 mouse offspring of three inbred strains of male mice（129/Sv, C3H and C57BL/6J） and female KM mice as test groups,and 129/Sv×KM,C57BL/6J × KM and C3H X KM,and F1 self-inbred lines of KM mice as control group were used to compare the hyperovulation results,activation rates and blastula rates of parthenogenetic embryos between test groups and control lines. [Result] There was no statistical difference in hyperovuation rates and activation rates of parthenogenetic embryo （P〉0.05） between three test groups and control （KM） group,but the parthenogenetic blastocyte rates of 129/SvX KM and C3H X KM were higher than that of others （P〈0.05）. And there were no differences in blastula rates between C57BL/6J × KM and KM × KM control lines. [Conclusion] The paternal genetic background of 129/Sv,C3H and C57BL/6J did not affect the hypovulation of hybrid F1 mice,but introduction of 129/Sv and C3H male mouse could improve embryo de velopment of hybrid F1 and increase blastocyst rate of parthenogenetic embryos.

关 键 词：小鼠 遗传背景 卵母细胞 孤雌发育 

分 类 号：Q953.3[生物学—动物学]