大豆异黄酮与葡萄籽原青花素对2型糖尿病大鼠抗氧化功能的影响  

Effects of Soybeanisoflavone and Grape Seed Proanthocyanidin on Anti-Oxidation Function in Rats with Type Two Diabetes

作　　者：梁佳琦[1] 朱翠凤[1] 李艳飞[1] 刘湘红[1] 杨湘霞[1] 张献共[1] 郭松超[1] 

机构地区：[1]广西医科大学公共卫生学院营养与食品卫生系,广西壮族自治区南宁市530021

出　　处：《中国全科医学》2008年第11期948-950,共3页Chinese General Practice

基　　金：深圳市科技局科研基金(200404096);广东省医学科研基金(A2006608);广西科学基金(桂科自0640088)

摘　　要：目的探讨大豆异黄酮(SIF)与葡萄籽原青花素(GSPC)对2型糖尿病大鼠抗氧化功能的影响。方法30只SD大鼠随机分成普食对照组(A组,6只,喂养基础饲料)和高脂饲料组(24只,高脂饲料喂养)。用链脲佐菌素将高脂饲料组大鼠制备成糖尿病大鼠,将模型制备成功的18只大鼠随机分成3组:糖尿病对照组(B组)、SIF+GSPC250mg·kg-1·d-1剂量干预组(C组)和SIF+GSPC50mg·kg-1·d-1剂量干预组(D组)。A组和B组大鼠用消毒后的自来水灌胃,C组和D组大鼠以两种剂量的SIF+GSPC分别灌胃。检测6周、10周后各组大鼠空腹血糖水平以及10周后大鼠血清超氧化物歧化酶(SOD)活性及还原型谷胱甘肽(GSH)、丙二醛(MDA)、一氧化氮(NO)水平。结果10周后C组和D组大鼠空腹血糖较B组均下降,但只有C组大鼠空腹血糖水平与B组相比,差别均有统计学意义(P<0.05)。C组大鼠血清SOD活性和GSH、MDA、NO水平与B组比较,差别均有统计学意义(P<0.05);D组大鼠SOD活性和NO水平与B组比较,差别有统计学意义(P<0.05)。结论大豆异黄酮协同葡萄籽原青花对于改善2型糖尿病大鼠的抗氧化能力有作用。Objective To observe the effect of soybeanisoflavone and grape seed proanthocyanidin（GSPC） on serum superoxide dismutase（SOD）, malonyldialdehede（MDA）, reduced glutathione hormone （GSH）, nitricoxide（NO）.Methods Thirty SD rats were randomly divided into a common control group（Group A, 6 rats, fed with common forage） and a high-fat forage group（fed with high-fat forage）. And then diabetes rats were prepared from the high-fat forage group using streptozocin and 18 successfully-prepared rats were randomized into three groups： Group B（a diabetes control group）, Group C（an intervention group given SIF＋GSPC in a dosage of 250 mg·kg^-1·d^-1 ） and Group D（an intervention group given SIF＋GSPC in a dosage of 50 mg·kg^-1·d^-1）. Group A and Group B were administered by gavage with sterilized tap water, while Group C and Group D with SIF＋GSPC in the two dosages respectively. Fasting blood sugar was detected after 6 weeks and 10 weeks respectively, and the changes of SOD, MDA, GSH and NO were determined after 10 weeks in all groups. Results After 10 weeks, a decrease of fasting blood sugar was observed in both Group C and Group D, but only Group C was significantly different from Group B in fasting blood sugar（P〈0.05）; there was significant difference in the SOD activity and the levels of GSH, MDA and NO between Group C and Group B（P〈0.05）; difference in the SOD activity and the level of NO between Group D and Group B was significant（P〈0.05 ）.Conclusion Soybean isoflavone with grape seed proanthocyanidin plays a role in improving the capability of anti-oxidation in rats with type Ⅱ diabetes.

关 键 词：大豆异黄酮 葡萄籽原花青素 糖尿病 2型 超氧化物歧化酶 还原型谷胱甘肽 丙二醛 一氧化氮 

分 类 号：R587[医药卫生—内分泌]