脐血来源的不同增殖能力的两种内皮祖细胞  

The proliferative potential differences between two types of endothelial progenitor cells from cord blood

作　　者：段华新[1] 卢光琇[2] 程腊梅[2] 

机构地区：[1]广州医学院附属广州市第一人民医院血液科,广东广州510180 [2]中南大学生殖与干细胞工程研究所人类干细胞国家工程研究中心,湖南长沙410078

出　　处：《中国热带医学》2008年第4期544-547,共4页China Tropical Medicine

基　　金：国家863项目基金(2003AA205181);国家973项目基金(00CB51010);国家自然科学基金重点项目(30030070);中国博士后项目基金(20070420762);湖南省自然科学基金(07JJ5034);广州市医药卫生科技项目(2007-YB-003)

摘　　要：目的比较两种脐血来源内皮祖细胞的增殖和抗凋亡能力。方法采用Annexin V/PI的凋亡检测比较两种细胞抵抗凋亡的能力;用增殖实验等方法比较两种内皮祖细胞的增殖特点;用集落形成实验比较两种细胞的集落形成能力。结果发现高增殖潜能内皮祖细胞(HPP-EPCs)显示比循环成血管细胞(CACs)具有更强的抗凋亡能力。CACs只能达到4～6个倍增,而HPP-EPCs能达到60多个倍增。CACs的单个细胞不能形成集落,HPP-EPCs的单个细胞中能在14d的培养期间形成了次级集落和三级集落。结论HPP-EPCs比CACs具有更强的抗凋亡能力、增殖能力和集落形成能力。Objective To compare the biological characteristics between the two types of endothelial progenitor cells. Methods Flow cytometry was performed to analyze the apoptosis in two types of EPCs induced by 48-hour serum-free culture. The growth curves were generated and the PDTs and CPDLs were calculated for the two types of cells and colony - forming assays were performed to compare the clonogenic potential between them. Results FACS showed that the incidence of apoptosis induced by 48 h serum-free culture in HPP - EPCs is less than CACs. The proliferation potential of HPP-EPCs was significant higher than CACs as the growth curve and MTT assay shown. The experiments of colony forming showed that no colony was formed in 960 individual cells from CACs, whereas about 45 percent of 960 individual cells formed colonies with more than 50 cells in a 2-week culture period and 5 tertiary colonies were formed in the total 96 individual cells from the secondary colonies. These data suggested that different hierarchies of EPCs from human umbilical cord blood including CACs and HPP - EPCs had been cultured and the later contained HPP - ECFC and LPP - ECFC. Conclusion HPP - EPCs possesses stronger ability of proliferation, clonogenic potential and resistance to apoptosis.

关 键 词：内皮祖细胞 增殖 凋亡 集落形成 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]