应用PCR-DGGE技术分析长江口低氧区的细菌群落组成  

Bacterial community composition in the Changjiang estuary's hypoxia studied by PCR-denaturing gradient gel electrophoresis

作　　者：刘敏[1,2] 王子峰[1] 朱开玲[1] 李洪波[1,3] 高绪敏[1,2] 邓兵[4] 肖天[1] 

机构地区：[1]中国科学院海洋研究所海洋生态与环境科学重点实验室,青岛266071 [2]中国科学院研究生院,北京100864 [3]国家海洋环境监测中心,大连116023 [4]华东师范大学河口海岸国家重点实验室,上海200062

出　　处：《高技术通讯》2008年第6期650-656,共7页Chinese High Technology Letters

基　　金：973计划(2006CB400604);国家自然科学基金(40376048);中国科学院研究生院科学与社会实践资助专项(创新研究类)资助

摘　　要：应用 PCR 结合变性梯度凝胶电泳(PCR-DGGE)技术对长江口外低氧区的细菌群落组成和优势菌群进行了分析。对25条 DGGE 条带进行了克隆、测序,所得到的细菌16SrDNA 基因 V3区序列进行了系统进化分析。结果显示这25条条带分别归属于4个细菌类群:变形菌门(Proteobacteria)、拟杆菌门(Bacteroides)、厚壁菌门(Firmicutes)和蓝细菌门(Cyanobacteria)。其中有16条分别与变形细菌亚群的γ-Proteobacteria 和δ-Proteobacteria 相似。时空分析发现,低氧水体的细菌群落组成与非低氧水体的组成不同,低氧水体包括变形菌门(Proteobacteria)、拟杆菌门(Bacteroides)、厚壁菌门(Firmicutes)和蓝细菌门(Cyanobac-teria),非低氧水体中没有发现蓝细菌门(Cyanobacteria)。并且8月份低氧水体中 Flavobac-teria 为优势菌群。The bacterial community composition and predominant bacteria in the hypoxia area of the Changjiang estuary were analyzed by PCR-denaturing gradient gel electrophoresis （DGGE）. Twenty-five DGGE bands were excised, cloned and sequenced. The phylogenetic analysis of the cloned bands shows that the bacterial phylotypes in the area are made up by four bacterial phyla： Proteobacteria, Bacteroides, Firmicutes and Cyanobacteria. Among these sequences, 16 are related to γ-Proteobacteria and δ-Proteobacteria. The spatiotemporal comparison reveals that bacterial community composition in the hypoxia is different from that in the non-hypoxia. Among the four phyla detected in the hypoxia, Proteobacteria, Bacteroides and Firmicutes are also found in non-hypoxia. In constrast, Cyanobacteria is present only in the hypoxia. The predominant bacteria are Flavobacteria in the hypoxia in August.

关 键 词：细菌群落组成 16S RDNA序列分析 PCR-DCA3E 长江口低氧区 

分 类 号：Q93[生物学—微生物学]