亚麻CAD基因克隆及序列分析  

Clone of Partial Sequence of Cinnamyl Alcohol Dehydrogenase Gene in Flax

作　　者：黄海燕[1] 王玉富[1] 薛召东[1] 邱财生[1] 郝冬梅[1] 

机构地区：[1]中国农业科学院麻类研究所,长沙410205

出　　处：《湖北农业科学》2008年第5期496-498,共3页Hubei Agricultural Sciences

基　　金：国家科技支撑计划项目(2006BAD13B04-2);农业部行业计划项目(nyhyzx07-018-03);农业部"948"项目[2006-G18(A)]

摘　　要：以亚麻(Linum usitatissimum L.)为材料,研究木质素代谢的关键酶亚麻肉桂醇脱氢酶基因序列。分离了高纯度的RNA,以RNA为模板,用简并引物以RT-PCR的方法,克隆了亚麻CAD基因cDNA部分序列,长度为477bp,编码159个氨基酸残基。此cDNA序列为亚麻CAD基因序列。The object of this study was to examine the sequence of flax CAD and investigate flax lignin biosynthesis pathway. High quality RNA was extracted from flax（Linum usitatissimum L.）and used as a template for cloning the cinnamyl alcohol dehydrogenase gene cDNA by RT-PCR with degenerate primers under gradient temperature. Colonies with target foreign DNA inserts was identified by PCR. Recombinant pGEM-T plasmid was identified by restriction digestion and PCR,also sequence analysis. Flax cinnamyl alcohol dehydrogenase gene cDNA partial sequence was 477 bp long, coding 159 amino acids. The result of the domain analysis showed that the flax CAD was a member of the CAD family.

关 键 词：亚麻 CAD基因 基因克隆 序列分析 

分 类 号：S563.2[农业科学—作物学] Q78[生物学—分子生物学]