弓形虫pcDNA3-ROP1 DNA疫苗免疫小鼠诱导体液免疫应答及保护性的初步观察  

On the Humoral and Protective Immune Responses Induced by pcDNA3-ROP1 DNA Vaccine of Toxoplasma gondii in Mice

作　　者：李薇[1] 刘波[1] 丑莉莉[1] 

机构地区：[1]北华大学医学检验学院,吉林吉林132013

出　　处：《北华大学学报（自然科学版）》2008年第3期239-240,共2页Journal of Beihua University（Natural Science）

基　　金：北华大学重点科研项目

摘　　要：目的观察弓形虫pcDNA3-ROP1DNA疫苗免疫BALB／C小鼠诱导其体内产生的体液免疫应答及抗虫感染的免疫保护作用．方法重组质粒用生理盐水稀释后，肌注免疫BALB／C小鼠．分别于免疫后5周和10周用ELISA法测定IgG抗体滴度；免疫鼠腹腔注射弓形虫速殖子攻击感染．结果经两次检测，均可测到特异性IgG抗体，且抗体的滴度随免疫时间的延长而增高；弓形虫速殖子腹腔攻击感染免疫组小鼠的平均存活时间较对照组延长，统计学有显著性差异（P〈0．01）．结论弓形虫pcDNA3-ROP1质粒DNA疫苗能诱导BALB／C小鼠产生特异性体液免疫应答及部分抗虫免疫保护作用．Objective To observe the humoral and protective immune responses induced by the pcDNA3-ROP1 DNA vaccine of Toxoplasma gondii in mice. Methods Large scale preparation of the recombinant plasmid pcDNA3-ROP1 was dissolved with normal saline and injected into BALB/C mice via muscles. After 5 and 10 weeks immunization, the IgG antibodies in serum from vaccinated mice were detected by ELISA. The vaccinated mice were challenged by RH tachyzoite of Toxoplasma gondii through injection. Results The detected results of two times showed that specific IgG antibody in serum from vaccinated mice could be detected and the fiter of IgG gradually rose with time prolonged. The average survival time of vaccinated mice was longer than that of control groups after mice were challenged with RH tachyzoite, there were remarkable differences （P 〈 0.01 ）. Conclusion The pcDNA3-ROP1 DNA vaccine of Toxoplasma gondii could elicit humoral and partial immune responses against the infection of Toxoplasma gondii.

关 键 词：弓形虫 pcDNA3-ROP1 DNA疫苗 体液免疫 

分 类 号：R382.5[医药卫生—医学寄生虫学]