内皮细胞提取和高效液相色谱－电喷雾飞行时间质谱联用预测玄参中的活性成分  

Endothelial cell extraction and HPLC-ESI/TOF MS analysis for predictingpotential bioactive components of Radix Scrophulariae

作　　者：祝艳斐[1] 毕志明[1] 刘承伟[1] 任美婷[2] 吴斐华[2] 李萍[2] 

机构地区：[1]中国药科大学生药学教研室,南京210009 [2]中国药科大学现代中药教育部重点实验室,南京210009

出　　处：《中国药科大学学报》2008年第3期228-231,共4页Journal of China Pharmaceutical University

基　　金：国家自然科学基金资助项目(No30600805);教育部新世纪优秀人才支持计划资助项目(NCET-06-0497)

摘　　要：目的:应用内皮细胞提取和高效液相色谱-电喷雾飞行时间质谱联用(HPLC-ESI/TOF MS)预测玄参的活性成分。方法:应用内皮细胞作为靶细胞,中药提取液中的活性成分可选择性地与其结合,洗去未结合的成分,用HPLC-ESI/TOF MS对细胞解离液中的成分进行分析。结果:从玄参提取液中检测出6个可与内皮细胞结合的成分,其中3个鉴定为安格洛苷C(angoroside C)、肉桂酸(cinnamic acid)和哈巴俄苷(harpagoside)。结论:细胞解离液中检测到的成分为玄参中与内皮细胞有结合作用的成分,本方法可用于预测中药中与内皮细胞相结合的活性成分。Aim： To predict potential bioactive components of Radix Scrophulariae using endothelial cell extraction and HPLC-ESI/TOF MS analysis. Methods： The potential bioactive components in the extract of Radix Scrophulariae could be selectively bound to the cells so that the unbound components were washed away by the sodium chloride solution, while the cell-combound components in the extract of denatured ceils were determined by HPLC-ESI/TOF MS analysis. Results： Six compounds in the extract of Radix Scrophulariae were found to selectively bound to the endothelial ceils, three of which were elucidated as angoroside C, cinnamic acid and harpagoside. Conclusion： This proposed approach may be applied to pinpoint bioactive candidates in traditional Chinese medicines.

关 键 词：内皮细胞提取 高效液相色谱-电喷雾飞行时间质谱联用法 玄参 活性成分 

分 类 号：R284[医药卫生—中药学]