Immunorecognition of estrogen and androgen receptors in the brain and thoracic ganglion mass of mud crab,Scylla paramamosain  

作　　者：Haihui Ye Huiyang Huang Shaojing Li Guizhong Wang 

机构地区：[1]Department of Oceanography, Xiamen University, Xiamen 361005, China

出　　处：《Progress in Natural Science:Materials International》2008年第6期691-695,共5页自然科学进展·国际材料（英文版）

基　　金：National Natural Science Foundation of China（Grant No.40776084,40406030）.

摘　　要：The brain and the thoracic ganglion of a crustacean can synthesize and secrete gonad-stimulating hormone(GSH)which stimulates the maturation of gonad.In the previous experiments,sex steroid hormones(estradiol,testosterone,progesterone,etc.)have been detected from the crustacean.However,the feedback regulation of sex steroid hormones on the brain and the thoracic ganglion of the crustacean has not been reported so far.In the present experiment,monoclonal antibodies were applied to investigate the immuno-recognition of estrogen receptor(ER)and androgen receptor(AR)in the brain and the thoracic ganglion mass of Scylla paramamosain.The results showed that the distribution of the immunopositive substances of ER and AR was extremely similar.They distributed in the protocerebrum,deutocerebrum and tritocerebrum of the brain,and mainly in protocerebrum.In the thoracic ganglion mass,immuno-positive substances distributed in the subesophageal ganglion,thoracic ganglion and abdominal ganglion,and mostly in subesophageal ganglion.Immunopositive substances of ER and AR mostly existed in the cytoplasm of neurons.The present study will provide mor-phological evidence for the origin and the evolution of ER and AR.In addition,the immunoreactivities of ER and AR suggested that the estrogen and androgen may be involved in the feedback regulation of crustacean neuroendocrine.The brain and the thoracic ganglion of a crustacean can synthesize and secrete gonad-stimulating hormone （GSH） which stimulates the maturation of gonad. In the previous experiments, sex steroid hormones （estradiol, testosterone, progesterone, etc.） have been detected from the crustacean. However, the feedback regulation of sex steroid hormones on the brain and the thoracic ganglion of the crustacean has not been reported so far. In the present experiment, monoclonal antibodies were applied to investigate the immunorecognition of estrogen receptor （ER） and androgen receptor （AR） in the brain and the thoracic ganglion mass of Scylla paramamosain. The results showed that the distribution of the immunopositive substances of ER and AR was extremely similar. They distributed in the protocerebrum, deutocerebrum and tritocerebrum of the brain, and mainly in protocerebrum. In the thoracic ganglion mass, immunopositive substances distributed in the subesophageal ganglion, thoracic ganglion and abdominal ganglion, and mostly in subesophageal ganglion. Immunopositive substances of ER and AR mostly existed in the cytoplasm of neurons. The present study will provide morphological evidence for the origin and the evolution of ER and AR. In addition, the immunoreactivities of ER and AR suggested that the estrogen and androgen may be involved in the feedback regulation of crustacean neuroendocrine.

关 键 词：Scylla paramamosain Estrogen receptor Androgen receptor BRAIN Thoracic ganglion mass IMMUNOCYTOCHEMISTRY 

分 类 号：Q959[生物学—动物学]