鲎抗脂多糖因子对晚期炎症因子高迁移率族蛋白-1体外效应的拮抗作用  

Antagonistic Effects of Limulus Anti-Lipopolysaccharide Factor on High Mobility Group Box-1 in vitro

作　　者：吴林青[1] 许伟群[1] 张秋玉[1] 胡海霞[1] 祝先进[1] 苏东辉[1] 

机构地区：[1]福建医科大学免疫学系,福州350004

出　　处：《福建医科大学学报》2008年第3期212-214,218,共4页Journal of Fujian Medical University

基　　金：福建省自然科学基金资助项目(C0410017);福建医科大学教授基金资助项目(JS06004)

摘　　要：目的观察鲎抗脂多糖因子(LALF)对小鼠巨噬细胞晚期炎症因子高迁移率族蛋白-1(HMGB1)功能的拮抗效应,探讨LALF对晚期内毒素血症动物的保护机制。方法用HMGB1刺激小鼠腹腔巨噬细胞,加入或不加LALF,迁移实验检测巨噬细胞趋化作用,ELISA检测培养上清液中肿瘤坏死因子(TNF)-α浓度。结果巨噬细胞迁移指数的增加呈HMGB1剂量依赖性,HMGB1刺激后巨噬细胞培养上清液TNF-α浓度显著增高。LALF与HMGB1预混合显著降低巨噬细胞的迁移指数和培养上清液TNF-α浓度。结论LALF可能通过抑制HMGB1而发挥其对晚期脓毒症动物的保护作用。Objective To investigate the effects of limulus anti-lipopolysaccharide factor（LALF） on the late proinflammatory cytokine of high mobility group box I（HMGB1）. Methods The primary mouse macrophages were cultured and stimulated by HMGB1. Macrophage migration was determined by the use of chemotaxis chamber. ELISA quantitated the culture supernatants for TNF-α production. LALF was added to explore the effects of HMGBI and TNF-α production in culture. Results The chemotactic index of macrophages and the concentration of TNF-α in the culture supernatants evidently increased in dose-dependent manner in HMGB1 groups and prominently decreased by the LALF co-incubated. Conclusion HMGB1 was firstly indicated in this study to be directly inhibited by LALF in late course of endotoxemia.

关 键 词：高迁移率族蛋白 脓毒症 鲎抗脂多糖因子 趋化作用 

分 类 号：R459.7[医药卫生—急诊医学]