荧光定量聚合酶链反应检测去颌下腺大鼠睾丸Bcl 2和Bax mRNA的改变  

Detection of Bcl 2 and Bax mRNA expression with fluorogenic quantitative PCR in rat testis after submandibular glands removed

作　　者：王晨阳[1] 马百坤[1] 侯林[1] 徐会茹[1] 黄祝[1] 蒋超[1] 黄宇烽[1] 姚兵[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所

出　　处：《生殖医学杂志》2008年第3期211-215,共5页Journal of Reproductive Medicine

基　　金：江苏省自然科学基金(BK2006135);全军"十一五"医药卫生科研基金(06H011)

摘　　要：目的观察切除颌下腺对大鼠睾丸Bax和Bcl 2 mRNA表达的影响。方法切除大鼠颌下腺,分别于术后14、28和42 d处死大鼠,提取睾丸总RNA,反转录,设计特异性引物和Taqman探针,荧光定量聚合酶链反应(PCR)检测Bax和Bcl 2 mRNA的改变。结果随着颌下腺切除时间的延长,实验组大鼠睾丸Bax和Bcl 2 mRNA的比值显著升高,与对照组比较,有显著性差异(P<0.01)。结论颌下腺切除大鼠睾丸Bax和Bcl 2 mRNA比值明显升高,提示颌下腺切除所致的大鼠睾丸生精细胞的凋亡可能是由Bax和Bcl 2介导的线粒体途径进行,但Fas/FasL是否参与该凋亡过程还有待进一步研究。Objective： To detect the changes of Bax and Bcl 2 mRNA expression in rat testis after the removal of submandibular glands. Methods： On day 14, 28 and 42 after operation （bilateral submandibular glands removed or shamoperated）, the levels of Bax and Bcl 2 mRNA in rat testis were examined by using fluorogenic quantitative RT-PCR. Results： Fluorogenic quantitative PCR showed that there was a significant increase in the ratio of Bax/Bcl 2 mRNA in rat testis on day 14, 28 and 42 after submanibular glands removed （P〈0.01）. Conclusions： The removal of submandibular glands altered the expression of Bax and Bcl 2 mRNA in rat testis and the resulted germ cell apoptosis of rat testis could be related to the mitochondria death pathway mediated by Bax and Bcl 2. The exact mechanism should be further explored.

关 键 词：荧光定量聚合酶链反应 颌下腺 睾丸 BCL 2 Bax 大鼠 

分 类 号：R339.21[医药卫生—人体生理学]