可溶性Jagged-1/Fc嵌合蛋白诱导淋巴结细胞向CD4^＋CD25^＋T细胞分化  

Soluble Jagged-1/Fc Chimera Protein Induces The Differentiation of Lymphonode Cells Into CD4^+CD25^+ T Cells

作　　者：邢飞跃[1] 刘静[2] 于哲[1] 季煜华[1] 

机构地区：[1]暨南大学组织移植与免疫研究中心 [2]暨南大学医学院,广州510632

出　　处：《生物化学与生物物理进展》2008年第6期676-683,共8页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30471635);广东省自然科学基金资(04010451,5006033).~~

摘　　要：直接用可溶性Jagged-1/Fc嵌合蛋白(Jagged-1/Fc)在体外诱导小鼠淋巴结细胞向CD4+CD25+T细胞分化.通过荧光标记单克隆抗体染色结合流式细胞术,观察不同剂量Jagged-1/Fc在不同时间对淋巴结细胞向CD4+CD25+T细胞分化的影响,观察Jagged-1/Fc诱导T细胞内细胞因子的变化;藉ELISA法检测Jagged-1/Fc诱导分化的T细胞分泌TGF-β1、IL-4和IL-10的水平.结果显示,超过500.0μg/L剂量的Jagged-1/Fc使CD4+CD25+T细胞百分比明显增高,诱导时间需要4～6天,抗Jagged-1单抗能抵消Jagged-1/Fc的诱导作用,用DAPT阻断Notch信号通路的活化也能抑制Jagged-1/Fc的诱导作用,Jagged-1/Fc诱导分化的T细胞培养上清中IL-4和IL-10的水平明显增高,TGF-β1无明显变化,胞内IL-4,IL-10,IL-2和TNF-α的水平也呈增高趋势.上述结果表明,可溶性Jagged-1/Fc嵌合蛋白在体外可诱导小鼠淋巴结细胞向CD4+CD25+调节性T细胞分化.A soluble Jagged-1/Fc chimera protein （Jagged-1/Fc） was directly used to induce the differentiation of lymphonode cells in mice into CD4^＋CD25^＋ regulatory T cells in vitro. A fluorescein-labeled monoclonal antibody staining combined with flow cytometry was applied to detect the effect of different doses of Jagged- 1/Fc on the differentiation of the lymphonode cells into CD4^＋CD25^＋ T cells at different time, and to measure intracellular cytokine changes of the T cells induced by Jaggde1/Fc. The level of TGF-β1, IL-4 and IL-10 secreted by the T cells that were induced by Jagged-1/Fc was determined by ELISA. The results showed that over 500.0 μg/L of Jagged-1/Fc led to the obvious enhancement of the proportion of CD4^＋CD25^＋ T cells within the day 4 to 6 of induction, which was abrogated with an anti-Jagged-1/Fc monoclonal antibody. This induction action of Jagged-1/Fc on CD4^＋CD25^＋ T cells was also inhibited by the block of a Notch signal pathway with N- [N- （3,5-difluorophenacetyl）-L-alanyl]-S-phenylglycine t-butyl ester （DAPT）. The level of IL-4 and IL-10 in the supernatant of T cell culture and their intracellular level were elevated by the induction of Jagged-1/Fc, and the level of TGF-β1 in the supematant was not altered. These findings suggest that a soluble Jagged-1/Fc chimera protein may induce the differentiation of mouse lymphonode cells into CD4^＋CD25^＋ regulatory T cells in vitro.

关 键 词：JAGGED-1 淋巴结细胞 调节性T细胞 分化 

分 类 号：Q26[生物学—细胞生物学] R392[医药卫生—免疫学]