人LCRG1基因启动子的鉴定与初步分析  

Cloning and Identification of Promoter of Human LCRG1 Gene

作　　者：谢海龙[1] 陈主初[2] 李金花[1] 

机构地区：[1]南华大学肿瘤研究所,衡阳421001 [2]中南大学肿瘤研究所,长沙410078

出　　处：《生物化学与生物物理进展》2008年第6期684-690,共7页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目(30572030).~~

摘　　要：Laryngeal carcinoma related gene 1(LCRG1)是一个喉癌候选抑瘤基因,为进一步深入研究其转录调控机制,应用5′RACE技术确定了该基因的转录起始位点,然后在对人LCRG1基因进行生物信息学分析的基础上,通过PCR定向克隆和酶切亚克隆策略,构建了11种含不同长度LCRG1启动子荧光素酶报告基因重组体.启动子活性分析表明,-169～+127区域的启动子活性最高.研究提示,LCRG1基因转录所必需的基因启动子序列在-169～+127范围内.Laryngeal carcinoma related gene 1 （LCRG1） is a candidate tumor suppressor gene of Laryngeal carcinoma. To further investigate its transcriptional regulation, the transcriptional start sites for LCRG1 gene have been identified by 5′ RACE （rapid amplification of cDNA ends） based on the bioinformation analysis of LCRG1. Then eleven luciferase expression vectors which contained potential human LCRG1 gene promoter were constructed. Luciferase reporter assay indicated that LCRG1 promoter region was mainly located in -169 ～＋ 127 region nearby the major transcriptional start site. These results suggested that the region （-169～＋ 127） includes an essential promoter for human LCRG1 gene transcription.

关 键 词：LCRG1 喉癌 启动子 转录调控 

分 类 号：Q74[生物学—分子生物学]