鸭疫里默氏杆菌强、弱菌株外膜蛋白的比较蛋白质组学研究  

Differential Proteomics of Outer Membrane Proteins Between Virulent and Avirulent Strains of Riemerella anatipestifer

作　　者：刘燕[1] 韦强[1] 鲍国连[1] 季权安[1] 

机构地区：[1]浙江省农科院畜牧兽医研究所,杭州310021

出　　处：《生物化学与生物物理进展》2008年第6期691-694,共4页Progress In Biochemistry and Biophysics

基　　金：浙江省科技厅重点项目(2008C22032;2005C12021;2005E60014).~~

摘　　要：应用双向电泳及质谱技术对血清2型鸭疫里默氏杆菌强毒株及其体外传代200代(RA200)的弱毒菌株的外膜蛋白进行比较蛋白质组学研究,借此分析鸭疫里默氏杆菌的外膜蛋白表达特点,研究差异表达蛋白与细菌毒力的关系.在实验中检测到血清2型鸭疫里默氏杆菌原代及其体外传代获得的弱毒菌株的外膜蛋白约表达60个蛋白质点(n=3),其中相差5倍以上3个.胶内酶解和肽质量指纹图谱分析后鉴定,W1为热休克蛋白Hsp20家族成员,W2、W3为转座酶,推测它们可能与里默氏杆菌的毒力密切相关.In order to identify virulence factors of the pathogen, the outer membrane proteins of virulent and avirulent strains of Riemerella anatipestifer were compared by a proteome analysis. Three protein spots differentially expressed between the two strains were observed by 2-DE gels, and were further analyzed using in gel tryptic digestion and peptide mass fingerprinting. Three proteins were identified. W1 was Hsp20, W2 and W3 were transposon. Although the exact role of these proteins has not been characterized, the exclusive expression in virulent strain may indicate that they play an important role in the pathogenesis of Riemerella anatipestifer infection. Although only two virulence factors identified, it opens a path to the further analysis of virulence factors of Riemerella anatipestifer.

关 键 词：鸭疫里默氏杆菌 外膜蛋白 双向电泳 毒力因子 

分 类 号：Q93[生物学—微生物学] S85[农业科学—兽医学]