高碘酸氧化肝素抑制β_2-整合素(Mac-1)介导的嗜中性粒细胞黏附(英文)  

RO-Heparin Inhibits β_2-Integrin(Mac-1)-mediated Neutrophils Adhesion

作　　者：陈志鸿[1] 静雅杰 王晓光[3] 曾宪录[1] 

机构地区：[1]东北师范大学遗传与细胞生物学研究所 [2]黑龙江省幼儿师范高等专科学校生物教研室,牡丹江157011 [3]长春师范学院生命科学学院,长春130032

出　　处：《生物化学与生物物理进展》2008年第6期695-702,共8页Progress In Biochemistry and Biophysics

基　　金：国家重点基础研究发展计划资助项目(2002CB513006);国家自然科学基金资助项目(30570927和30570928).~~

摘　　要：已有的研究结果表明,肝素可以作为β2-整合素(Mac-1)的配体抑制炎症过程中Mac-1介导的嗜中性粒细胞与血管内皮细胞的黏附.通过选择性化学修饰方法制备了具有低抗凝血活性的高碘酸氧化-硼氢化钠还原肝素(RO-肝素),系统地研究了它对Mac-1介导的嗜中性粒细胞黏附的抑制作用.结果表明,显著失去抗凝血活性的RO-肝素仍能有效地抑制Mac-1介导的嗜中性粒细胞与ICAM-1重组蛋白、转染ICAM-1 cDNA的COS-7细胞和人脐静脉内皮细胞黏附.为深入阐明拮抗Mac-1介导的白细胞黏附的分子机制和筛选抗炎症药物提供了有价值的实验证据.The adhesion of leukocytes to vascular endothelium is crucial for the generation of inflammatory responses. The selectins and β2-integrin （Mac-1） play a major role in the process. Recently, it was reported that RO-heparin can inhibit selectin-mediated leukocyte adhesion. The effect of RO-heparin on the Mac-1-mediated neutrophils adhesion were further tested. The results showed that RO-heparin could effectively inhibit neutrophils binding to ICAM-1, adhering to COS-7 cells expressing human ICAM-1, and adhering to human umbilical vein endothelial cells （HUVECs） under static and flow conditions. The findings suggest that the effect of RO-heparin on leukocyte adhesion is mainly due to its inhibition on the interaction between selectins or Mac-1 and their ligands and that RO-heparin might be useful in preventing inflammation diseases.

关 键 词：高碘酸氧化肝素 β2-整合素(Mac-1) 嗜中性粒细胞 人脐静脉内皮细胞 

分 类 号：Q26[生物学—细胞生物学] X520.322.5[环境科学与工程—环境工程]