我国引进条斑星鲽群体遗传多样性的AFLP分析  

Genetic diversity of introduced barfin flounder(Verasper moseri) populations by AFLP analysis

作　　者：马洪雨[1,2] 陈松林[2] 田永胜[2] 季相山[2] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266003 [2]中国水产科学研究院黄海水产研究所,农业部海洋渔业资源可持续利用重点开放实验室,山东青岛266071

出　　处：《水产学报》2008年第3期321-326,共6页Journal of Fisheries of China

基　　金：国家“八六三”高技术研究发展计划(2006AA10A403);山东省泰山学者建设工程专项经费资助;上海高校水产养殖学E-研究院项目(E030090)

摘　　要：应用AFLP技术对我国条斑星鲽引进群体（烟台、大连和莱州）共63尾个体的遗传多样性及遗传变异进行分析，计算了3个群体间的遗传相似性指数和遗传距离，并构建了UPGMA系统发生树。10个引物组合在3个群体中共扩增到827个位点，大小位于50-700bp。每个引物组合扩增到的多态性条带在8到37条之间不等，平均为17．9个。3个群体的多态性位点比例分别为29．14％、15．60％和20．31％；Shannon多样性指数分别为0．1799、0．0949和0．1231；Nei基因多样性指数分别为0．1225、0．0658和0．0848。3个条斑星鲽引进群体的遗传多样性水平，烟台引进群体最高，莱州引进群体次之，大连引进群体最低，但总体水平均较低。烟台引进群体与大连引进群体间的遗传距离最大为0．0230，莱州引进群体与大连引进群体间的遗传距离最小为0．0129。3个引进群体间的遗传分化系数（Gst）为0．219，表明3个引进群体之间发生了一定程度的分化。Amplified fragment length polymorphism （AFLP） technology was used to evaluate the genetic diversity of three introduced populations （Yantai, Dalian and Laizhou） of introduced barfin flounder （Verasper moseri）. The genetic distance and identity index were calculated, and according to them, the UPGMA dendrogram was constructed. A total of 827 loci ranged from 50 - 700 bp were detected using 10 primer combinations, and 8 - 37 polymorphic loci were detected per primer combination. The percentage of polymorphic loci of three introduced populations of barfin flounder were 29. 14%, 15. 60% and 20. 31%, respectively. The Shannon＇s information index were 0. 1799, 0. 0949 and 0. 1231, respectively, and the Nei gene diversity were 0. 1225, 0. 0658 and 0, 0848, respectively. The total genetic diversity level of three introduced populations was low, of which YT introduced population was the highest, LZ introduced population was lower, and DL introduced population was the lowest. The genetic distance between YT introduced population and DL introduced population was the highest （0. 0230）, whereas, the genetic distance between LZ introduced population and DL introduced population was the lowest （0. 0129）. The coefficient of gene differentiation （Gst） value was 0. 219, showing a certain extent of differentiation among three introduced populations of barfin flounder.

关 键 词：条斑星鲽 遗传多样性 扩增片断长度多态性 遗传分化 

分 类 号：S917[农业科学—水产科学] Q958[生物学—动物学]