病原性河流弧菌对青石斑鱼体表黏液黏附特性的研究  

Characteristics of adherence of pathogenic Vibrio fluvialis to the skin mucus of Epinephelus awoara

作　　者：王晓露[1] 邹文政[1] 鄢庆枇[1] 郭国军[1] 陈强[1] 

机构地区：[1]集美大学水产学院,福建省高校水产科学技术与食品安全重点实验室,福建厦门361021

出　　处：《水产学报》2008年第3期441-447,共7页Journal of Fisheries of China

基　　金：国家“八六三”高技术研究发展计划(2002AA639600);福建省科技项目(2006F5066);福建省高校水产科学技术与食品安全重点实验室开放基金(2007J101)

摘　　要：采用3H-TdR同位素示踪法研究了河流弧菌对不同处理后的青石斑鱼表皮黏液的黏附作用。结果显示:青石斑鱼表皮黏液经40、60和80℃热处理10min后河流弧菌的黏附率出现显著性升高,而100℃处理10min则能极显著降低河流弧菌的黏附作用;蛋白酶K和胰蛋白酶处理黏液对河流弧菌的黏附作用没有显著影响,高碘酸处理则能极显著提高河流弧菌的黏附率;8种碳水化合物中的葡萄糖、甘露糖、甘露醇、麦芽糖能极显著促进河流弧菌的黏附作用,乳糖、果糖及蔗糖能显著性促进河流弧菌的黏附作用;半乳糖则没有显著性影响;高浓度的细胞表面提取物能显著抑制河流弧菌的黏附作用,但较低浓度的细胞表面提取物反而有一定的促进作用;青石斑鱼表皮黏液经Sephadex G-100分离得到3个洗脱峰,河流弧菌对第1个洗脱峰的黏附作用最强。研究结果表明,病原性河流弧菌对青石斑鱼表皮黏液有较强的黏附作用;青石斑鱼表皮黏液中存在特异性黏附受体;黏液中的主要黏附受体是热稳定性较高、耐蛋白酶K和胰蛋白酶的大分子物质。In order to understand the adhesion characteristic of pathogenic Vibrio fluvialis to the skin mucus of Epinephelus awoara, isotope tracer method was introduced to investigate the bacterial adhesion of the pathogen to the mucus after different treatment. A significant enhancement in the adhesion rate of V. fluvialis to the skin mucus was observed after heating the mucus at 40, 60 and 80 ℃ for 10 min. However, an extremely significant inhibition in the bacterial adhesion was observed when the mucus had been heated at 100 ℃ for 10 min. No remarkable effect on the bacterial adhesion was found after treating the mucus with protease K and trypsin, whereas the bacterial adhesion was extremely significantly enhanced by treating the mucus with periodic acid; It was observed that pretreating the mucus with glucose, mannose, mannitol and maltose enhanced the bacterial adhesion extremely significantly, and other 3 carbohydrates enhanced the bacterial adhesion significantly; while galactose could not enhance the bacterial adhesion significantly. Surface extract of V. fluvialis with high concentration inhibited the bacterial adhesion significantly, whereas the surface extract with relative low concentration enhanced the bacterial adhesion. 3 protein peaks were obtained by fractionating the mucus with a Sephadex G- 100 column, and the strong adhesion was observed to the first peak. The results indicated that： pathogenic V. fluvialis adhere to the skin mucus of E. awoara strongly; there were specific adhesive receptors within the mucus; the adhesive receptors were likely to be macromolecules with relative stability to heating and protease.

关 键 词：河流弧菌 青石斑鱼 表皮黏液 黏附作用 

分 类 号：Q949.321[生物学—植物学]