HBV核心基因、γ-IFN基因重组质粒联合免疫BALB/c小鼠的实验研究  

The Immune Effective of Hepatitis B Viral Core Gene, IFN-γ Gene Expressing Recombinant Plasmids Co-combine Immunize BALB/c Mouse

作　　者：韦嘉[1] 姚集鲁[2] 杨林[2] 姚春斓[2] 江元森[2] 

机构地区：[1]昆明医学院第一附属医院感染疾病科,云南昆明650032 [2]中山大学附属第三医院感染疾病科,广东广州510630

出　　处：《昆明医学院学报》2008年第3期61-65,共5页Journal of Kunming Medical College

基　　金：广东省自然科学基金面上项目资助(990108);昆明医学院第一附属医院博士基金资助(BS2004)

摘　　要：目的应用表达乙型肝炎病毒(HBV)核心基因(c)与小鼠γ-干扰素(IFN-γ)基因产物的两种重组质粒联合免疫BALB/c小鼠,探讨联合使用表达细胞因子基因重组质粒对DNA免疫的影响及应用价值.方法将HBVc基因与IFN-γ基因重组质粒直接注射到BABL/c小鼠四头肌内,分析免疫后小鼠T细胞增殖、NK细胞杀伤活性及细胞因子释放等免疫应答效应.结果联合γ-IFN基因与HBVc基因免疫小鼠,对T细胞的增殖,NK细胞的杀伤活性及Th1细胞因子的产生等均有显著的增强效应.结论HBVc基因联合γ-IFN基因免疫BALB/c小鼠后,较之单独应用HBVc基因免疫可显著提高抗病毒免疫应答效应,值得进一步开展临床前应用研究.Objective Using hepatitis B viral core gene and interferon-gamma （IFN-γ） gene recombinant plasmid co-combine immunize BALB/c mouse to explove the application values of the cytokine expressing plasmids-mediated immune response. Methods Through direct inject recombinant plasmids DNA into quadriceps muscle of BALB/c mouse, to analysis immune effective including T lymphocytes proliferation, killing activity of natural killer cells and cytokines release. Results IFN- γ expressing plasmids enhance the T lymphocytes proliferation, killing activity of natural killer cells and IFN-γ release than only alone immune with hepatitis viral core gene expressing plasmid significantly. Conclusions After co-combination with IFN-γ expressing plasmid, the immune response which induced by hepatitis B core gene recombinant plasmid augmented obviously. Application of IFN- γ expressing plasmid deserves to undertake pre-clinical explore.

关 键 词：乙型肝炎病毒 γ-干扰素 DNA免疫 

分 类 号：R392-33[医药卫生—免疫学]