系统性红斑狼疮患者外周血单个核细胞增殖诱导配体及其受体的表达  

Expression of proliferation inducing figand and its receptors in the peripheral blood mononuclear cells of patients with systemic lupus erythematosus

作　　者：胡绍先[1] 孔芳[1] 熊金河[1] 萧文泽[1] 雷小妹[1] 张胜桃[1] 何培根[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院风湿免疫科,武汉430030

出　　处：《中华风湿病学杂志》2008年第6期400-403,共4页Chinese Journal of Rheumatology

基　　金：湖北省自然科学基金资助项目（2007ABA110）

摘　　要：目的 探讨系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMC）中增殖诱导配体（APRIL）及其受体B细胞成熟抗原（BCMA）、跨膜激活剂及钙调亲环素配体相互作用分子（TACI）mRNA的表达和意义。方法 应用实时定量聚合酶链反应（real—time PCR）技术，检测66例SLE患者及25名正常人PBMC中APRIL及其受体mRNA的表达，采用2-AACT对目标基因的表达量进行评估。结果 SLE活动组和缓解组的APRIL mRNA、BCMA mRNA和TACI mRNA的表达水平均明显高于正常对照组（均P〈0．01）；且SLE活动组的APRILmRNA和TACImRNA的表达水平显著高于SLE缓解组（分别P〈0．01，P〈0．05），BCMA mRNA的表达在SLE活动组与缓解组之间比较差异无统计学意义；此外，APRILmRNA和TACImRNA在SLE肾损组的表达显著高于非肾损组（均P〈0．01）。结论 SLE患者PBMC中APRIL及其受体的表达水平增高，可能在SLE发病机制中发挥重要作用。Objective To investigate the mRNA expression of a proliferation inducing ligand （APRIL） and its receptors including B cell maturation antigen （BCMA）, transmembrane activator, calcium modulator and cyclophilin ligand interactor （TACI） in the peripheral blood mononuclear cells （PBMCs） of patients with systemic lupus erythematosus （SLE）. Methods APRIL mRNA,BCMA mRNA and TACI mRNA in PBMCs were detected by real-time quantitative PCR in 66 SLE patients and 25 normal controls. Gene expression level was measured as 2-AACT. Results The expression levels of APRIL mRNA,BCMA mRNA and TACI-mRNA were significantly increased in both active SLE group and stable SLE group compared with those in the normal controls （P〈0.01 for all）. The expression levels of APRIL mRNA and TACI mRNA in active SLE group were significantly higher than those in stable SLE group （P〈0.01, P〈0.05, respectively ）. But there was no significant difference in the expression levels of BCMA mRNA between the SLE stable and active groups. Beside, the expression levels of APRIL mRNA and TACI mRNA were significantly increased in patients with lupus nephritis （LN） compared to patients with non-LN （P〈0.01 for all）. Conclusion The expression levels of APRIL and its receptors are significantly elevated in SLE patients. It may suggest that APRIL and its receptors play an important role in the pathogenesis of SLE.

关 键 词：红斑狼疮 系统性 增殖诱导配体 B细胞成熟抗原 跨膜激活剂及钙调亲环素配体相互作用分子 

分 类 号：R593.241[医药卫生—内科学]