高压氧对异基因骨髓移植后aGVHD影响的实验研究(英文)  

Effect of Hyperbaric Oxygen on Acute Graft-Versus-Host Disease after Allogeneic Bone Marrow Transplantation

作　　者：宋晓宇[1] 孙鲁宁[1] 郑宁宁[1] 张海鹏[1] 

机构地区：[1]中国医科大学基础医学院病理生理教研室,辽宁沈阳110001

出　　处：《中国实验血液学杂志》2008年第3期623-626,共4页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目,编号39570778~~

摘　　要：本研究探讨高压氧(HBO)在抗异基因骨髓移植后aGVHD和提高生存率中的作用及机制。给受致死量照射的C57BL/6受鼠注入供鼠BALB/c骨髓与脾淋巴细胞的混合液,分别给与高压氧、环孢素A、氨甲蝶呤处理。用流式细胞仪检测脾T细胞及其亚群和黏附分子CD3+、CD4+、CD8+、CD4+CD11a+、CD4+CD18+、CD8+CD11a+、CD8+CD18+的表达,用ELISA和RT-PCR法检测细胞因子IL-2、IL-4、IL-10和TNFα的表达。结果表明:HBO组小鼠11天的活存率明显高于异基因移植组及CsA+MTX组;HBO和CsA+MTX可明显降低脾组织中CD3+、CD4+、CD8+、CD4+CD11a+、CD4+CD18+、CD8+CD11a+、CD8+CD18+的表达(p<0.05);HBO组小鼠血清IL-2和TNFα的浓度及脾组织中IL-2和TNFα mRNA的含量都低于异基因骨髓移植组(p<0.05),但高于CsA+MTX组;HBO组小鼠脾组织中IL-4和IL-10 mRNA的含量显著高于异基因骨髓移植组和CsA+MTX组(p<0.05)。结论:高压氧在对抗aGVHD和提高活存率方面优于环孢素A和氨甲蝶呤,其抗aGVHD的作用机制与调节促炎/抑炎细胞因子的分泌和黏附分子的表达及抑制T淋巴细胞作用有关。The objective of this study was to investigate the function and mechanism of hyperbaric oxygen （HBO） in antagonizing acute graft-versus-host disease （aGVHD） and improving the rate of survival. The lethally irradiated C57BL/6 recipients were injected with bone marrow and lymphocyte of spleen from BALB/c donors and were treated with HBO, cyclosporine A （CsA） and methotrexate （MTX）. T lymphocytes and subsets, adhesion molecules and cytokines were detected by flow cytometry, ELISA and RT-PCR respectively. The results showed that the survival rate in HBO group was much higher than that in allogenetic bone marrow transplantation （allo-BMT） group and CsA ＋ MTX group ; the numbers of CD3 ^＋ , CD4 ^＋ , CD8 ^＋ , CD4 ^＋ CD11 a ^＋ , CD4 ^＋ CD18 ^＋ , CD8 ^＋ CD11 a ^＋ , CD8 ^＋ CD18 ^＋ lymphocytes in spleen were decreased markedly by HBO and CsA ＋ MTX（p 〈0.05） ; the levels of IL-2 and TNFα mRNA and their serum concentrations in HBO group were much lower than those in allo-BMT group but were higher than those in CsA ＋ MTX group; the levels of IL-4 and IL-10 mRNA in HBO group were much higher than those in allo-BMT group and CsA ＋ MTX group. It is concluded that HBO has more remarkable advantage in improving the rate of survival than CsA ＋ MTX, its mechanism of anti-aGVHD is tightly correlated with the transform of T cell and its subsets and the expression of adhesion molecules and cytokines.

关 键 词：高压氧 异基因骨髓移植 急性移植物抗宿主病 T淋巴细胞 细胞因子 

分 类 号：R459.6[医药卫生—治疗学] R457.7[医药卫生—临床医学]